Application of circactin compound in the preparation of marine fouling biological control agent
A technology of biological control agent and cyclin, which is applied in the direction of biocide, biocide-containing paint, plant growth regulator, etc., to achieve the effects of environmental friendliness, high safety and good application prospects
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Embodiment 1
[0026] Experimental group: Dissolve a quantitative amount of cyclin compound in methanol with a solution concentration of 282.6 μg / mL, add 1 mL of the solution to a petri dish with a diameter of 6 cm, and make it evenly cover the bottom of the petri dish. After the solvent is completely evaporated, the content of the cyclin compound coated on the bottom of the petri dish is 10 μg / cm 2 . Add 13 mL of filter-sterilized seawater.
[0027] Control group: Add 1 mL of solvent methanol to make it evenly distributed on the bottom of the culture dish. After the solvent is completely evaporated, add 13 mL of filtered and sterilized seawater.
[0028] Blank group: Add 13 mL of filtered and sterilized seawater.
[0029] Four parallel samples were set up in each of the experimental group, the blank group and the control group. During the attachment test, the reticulated barnacle Venus larvae were randomly assigned to the blank group, the control group, and the experimental group. About ...
Embodiment 2
[0033] Experimental group: Dissolve the cyclin compound in methanol to prepare a solution with a concentration of 28.26 μg / mL. Add 1 mL of this solution to a petri dish with a diameter of 6 cm and make it evenly cover the bottom of the petri dish. After the solvent is completely evaporated, the content of circactin compound coated on the bottom of the petri dish is 1.0 μg / cm 2 . Add 13 mL of filter-sterilized seawater.
[0034] Control group: Add 1 mL of solvent methanol to make the solution evenly distributed on the bottom of the petri dish. After the solvent is completely evaporated, add 13 mL of filtered and sterilized seawater.
[0035] Blank group: Add 13 mL of filtered and sterilized seawater.
[0036] Determination of the number of larvae: Take the water body containing the larvae of the emerald mussel vectoli from the nursery pond and concentrate it with sieve silk. Take 50 mL each for three times, add 1-2 drops of formalin solution to kill the larvae, and then cou...
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