A method for the determination of antibiotic residues

Abstract

The patent of the present invention relates to the technical field of antibiotic detection, and discloses a method for determining antibiotic residues. By heating a high-voltage pulsed electric field in sample pretreatment and designing the crushing step, oxytetracycline, tetracycline, and aureomycin are detected during detection. The prime peak shape is good, and it is well separated within 10 minutes; the linear relationship is good, and the correlation coefficients are all above 0.9992; the RSDs of three different concentrations of oxytetracycline, tetracycline, and aureomycin are below 5%. good; the recoveries of standard additions were greater than 85%; the detection limit of oxytetracycline was 3.3×10 ‑3 μg / ml, the detection limit of tetracycline is 3.8×10 ‑3 μg / ml, the detection limit of aureomycin is 4.6×10 ‑3 μg / ml, the detection limit is low; therefore, this method can meet the simultaneous, rapid, accurate and large-scale detection of oxytetracycline, tetracycline and chlortetracycline.

Description

A method for determining antibiotic residues technical field Patent of the present invention relates to the technical field of antibiotic detection, specifically, relate to a kind of method for measuring antibiotic residues. method. Background technique Tetracycline antibiotics (Tetracyclines, TCs) are a class of broad-spectrum antibacterial drugs, mainly containing tetracycline (TC), Chlortetracycline (CTC), Oxytetracycline (OTC), etc., due to their broad antibacterial spectrum and high price Low price is widely used in livestock breeding. If residents consume animals containing TCs antibiotics for a long time or in large quantities at one time Sexual foods can cause dysbiosis, gastrointestinal reactions, cause liver damage, and affect the development of teeth and bones. At present, the detection method of tetracycline antibiotic residues mainly contains microorganism method, immunoassay, thin layer chromatography method, capillary electrophoresis, electrochemi...

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to provide a fast, sensitive, high accuracy, good separation effect, can simultaneously measure OTC, TC, CTC residual and be suitable for the method for the determination of three kinds of tetracycline antibiotic residues in animal food, which is suitable for large batch sample detection, It aims to solve the problems of difficult separation of oxytetracycline and aureomycin and high detection limit existing in the prior art

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