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Enteromycodermitis animal model as well as construction method and application thereof

A construction method and intestinal mucositis technology, which can be applied in pharmaceutical formulations, compound screening/testing, preparations for in vivo experiments, etc., can solve the problems that the protein does not have binding ability, and the animal models of rats and mice cannot reflect the efficacy of drugs, etc. Achieve high application value

Active Publication Date: 2020-12-11
上海澎立生技医药研究有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, due to differences in genes, especially protein structures, between humans and mice, antibodies against humans often do not have the ability to bind to mouse proteins, and cannot show drug efficacy in traditional mouse or mouse models.

Method used

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  • Enteromycodermitis animal model as well as construction method and application thereof
  • Enteromycodermitis animal model as well as construction method and application thereof
  • Enteromycodermitis animal model as well as construction method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] In this example, 10 cynomolgus monkeys aged 3-5 years and weighing 2-5 kg ​​were divided into two groups according to body weight by using Biobook software, namely control group and model group, with 5 monkeys in each group. Irinotecan hydrochloride was prepared into a solution with physiological saline.

[0056] From day 0, the control group received normal saline as the control group; the model group received daily 30 mg / kg irinotecan hydrochloride for 5 consecutive days. The administration method is intravenous infusion, the injection volume is 10mL / kg, and the infusion time is 30min. The duration of the experiment was 14 days.

Embodiment 2

[0058] This example constructs an animal model of intestinal mucositis. The difference between its construction method and the model group construction method in Example 1 is that the cynomolgus monkey with a body weight of 2-5 kg ​​is replaced with a cynomolgus monkey with a body weight of 6-8 kg, and other conditions are the same. constant.

Embodiment 3

[0060] In this example, an animal model of intestinal mucositis was constructed. The difference between its construction method and the model group construction method in Example 1 was that the daily dose of irinotecan hydrochloride was 50 mg / kg, and other conditions remained unchanged.

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Abstract

The invention relates to an enteromycodermitis animal model as well as a construction method and application thereof. The construction method comprises the step of performing irinotecan administrationon old world monkeys to obtain the enteromycodermitis animal model. The irinotecan is used for the old world monkeys for the first time, the enteromycodermitis animal model is constructed, the modelis similar to a human enteromycodermitis patient, and symptoms such as a delayed diarrhea reaction, emesis, salivation, anorexia and activity decline are also accompanied. The animal model has the advantages of closer system affinity and cross-reactivity to specific biomolecules for humans due to high homology with the humans. The model meets a series of evaluation requirements for safety, effectiveness and the like of the irinotecan side effect medicine, and has extremely high application value.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to an animal model of intestinal mucositis and its construction method and application, in particular to an animal model capable of well simulating human intestinal mucositis, its construction method and application. Background technique [0002] Irinotecan, or CPT-11, is a very commonly used antitumor drug, which has shown very broad-spectrum and effective antitumor effects in both in vivo and in vitro studies. Moreover, irinotecan itself and its metabolites still have a good effect on tumors expressing multiple drug resistance genes. It is often used clinically to effectively treat small cell and non-small cell lung cancer, cervical cancer, ovarian cancer, gastric cancer, metastatic colorectal cancer, malignant lymphoma, etc. [0003] However, patients using irinotecan as a chemotherapy drug have a higher incidence of side effects of oral and gastrointestinal mucositis. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/02A61K49/00
CPCA01K67/02A61K49/0008A01K2207/20A01K2227/106A01K2267/03
Inventor 戴飞红俞诚杨平王自强成艳朱莉芸杨楠段继峰
Owner 上海澎立生技医药研究有限公司