Application of antibody CD29 in identification and sorting of human-placenta-chorion-avillosum-derived mesenchymal stem cells

A technology of mesenchymal stem cells and human placenta, applied in animal cells, vertebrate cells, cell dissociation methods, etc., can solve the dysfunction of human placental smooth chorionic mesenchymal stem cells Stem cell difficulties and other problems, to achieve rapid and accurate identification, good detection stability
CN112063581AInactive Publication Date: 2020-12-11CHENGDU QINGKE BIOTECH
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
CHENGDU QINGKE BIOTECH
Publication Date
2020-12-11
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses an application method of CD29 in identification and sorting of human-placenta-chorion-avillosum-derived mesenchymal stem cells. According to the application method disclosed bythe invention, the human-placenta-chorion-avillosum-derived mesenchymal stem cells (PMSCs) are firstly marked with a variety of cell surface markers separately, then, detection is carried out by using a flow cytometer to survey qualified rate and stability of flow-type detection marked with all the markers, and it is discovered that the expression of a marker CD90 in a universal standard is unstable, and detections are all qualified after the PMSCs are marked with the CD29; and through repeated test, it is discovered that the CD29 has good detection stability, the qualified rate of detectionis 100%, thus, the CD29 has a good and stable identification effect on the PMSCs and can be applied to rapid and accurate identification on the PMSCs instead of the CD90.
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Description

technical field

[0001] The invention belongs to the technical field of identification of mesenchymal stem cells. Background technique

[0002] In the basic research or clinical research of biomedicine, when analyzing the cell number and function changes of a specific cell group, it is necessary to separate this group of cells from other cells. At this time, it is necessary to separate them according to different cell surface markers. separate. Cell surface markers refer to membrane proteins embedded in the lipid bilayer structure of cell membranes, including membrane antigens, membrane receptors and other molecules. CD molecules (Cluster of Differentiation, differentiation clusters or differentiation groups, also called leukocyte differentiation antigens) are One of the most commonly used cell surface markers refers to the application of monoclonal antibody identification-based methods to identify the same differentiation antigen recognized by monoclonal antibodies from dif...

Claims

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