A kind of foot-and-mouth disease subunit vaccine and its preparation method and application
A subunit vaccine, foot-and-mouth disease technology, applied in the field of genetic engineering, can solve the problems of inability to replicate, non-infectious, etc., and achieve the effects of extensive cross-protection, high immune efficacy, and good antigen matching.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0056] Embodiment 1O type foot-and-mouth disease subunit vaccine
[0057] 1. Preparation of O-type foot-and-mouth disease structural protein composition
[0058] 1. Construction of recombinant expression vector for structural protein of foot-and-mouth disease virus
[0059] (1) Design the gene sequence THS encoding the fusion tag protein composed of the following elements in series, wherein T is the nucleotide sequence of the translation initiation region, H is the nucleotide sequence encoding the histidine tag, and S is the nucleotide sequence encoding the tag containing The nucleotide sequence of Saccharomyces cerevisiae small ubiquitin-like modification protein (SUMO); the nucleotide sequence of THS is shown in SEQ ID NO:21.
[0060] (2) The above-mentioned THS gene sequence was concatenated in sequence with the genes VPO, VP3, and VP1 encoding the structural proteins of the O / 17002 strain, respectively, to form three fusion gene sequences THS-VPO, THS-VP3, and THS-VP1. W...
Embodiment 2
[0087] Embodiment 2 Type A foot-and-mouth disease subunit vaccine
[0088] 1. Preparation of type A foot-and-mouth disease structural protein composition
[0089] The preparation process of the type A foot-and-mouth disease structural protein composition is the same as the preparation process of the type O foot-and-mouth disease virus-like particle in Example 1. Wherein, the amino acid sequence of the A / GDMM / 2013 strain structural protein VPO is shown in SEQ ID NO: 11, and the nucleotide sequence is shown in SEQ ID NO: 12; the A / GDMM / 2013 strain structural protein The amino acid sequence of VP1 is shown in SEQ ID NO: 13, and the nucleotide sequence is shown in SEQ ID NO: 14; the amino acid sequence of the structural protein VP3 of the A / GDMM / 2013 strain is shown in SEQ ID NO: 17, and the core The nucleotide sequence is shown in SEQ ID NO: 18; the amino acid sequence of the mutated structural protein VP1 of the A / GDMM / 2013 strain is shown in SEQ ID NO: 15, and the nucleotide s...
Embodiment 3
[0093] Embodiment 3 foot-and-mouth disease subunit vaccine animal immune challenge evaluation
[0094] The foot-and-mouth disease structural protein composition Re / O / 17002 / K3118R+K1209R prepared in Example 1 was mixed with an equivalent amount of 206 adjuvant to obtain the O-type foot-and-mouth disease subunit vaccine; the foot-and-mouth disease structural protein composition Re prepared in Example 2 / A / GDMM / 2013 / K3118R+K1209R is mixed with an equal amount of 206 adjuvant to obtain a type A foot-and-mouth disease subunit vaccine; the foot-and-mouth disease structural protein composition Re / O / 17002 / K3118R+K1209R prepared in Example 1 and the embodiment 2 The prepared foot-and-mouth disease structural protein composition Re / A / GDMM / 2013 / K3118R+K1209R was mixed in equal amounts, and then an equal amount of 206 adjuvant was added and mixed to obtain the O / A bivalent foot-and-mouth disease subunit vaccine.
[0095] 1. Neutralizing antibody detection
[0096] 10-week-old experimenta...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More