Resin embedding device and automatic liquid preparation method

Abstract

The invention relates to the technical field of sample preparation, and discloses a resin embedding device. The device comprises a freezer and an operation table, wherein a plurality of resin embedding instruments are arranged in the freezer; an upper computer, an electrical control box and a waste liquid bottle are arranged in the operation table; the resin embedding instrument comprises a box body, a handle is arranged on the box body; a plurality of peristaltic pumps and solution bottles are arranged in the box body; the plurality of peristaltic pumps are connected with the solution bottlesthrough a plurality of sections of liquid conveying pipelines; a sample bottle is also arranged in the box body; the operation table further comprises a power supply socket, a power-off protection circuit breaker and an external power socket. and the solution bottles comprise an aqueous solution bottle, a water / ethanol mixed solution bottle, an ethanol / resin mixed solution bottle, a resin solution bottle, a PBS solution bottle and an ethanol solution bottle.

Description

technical field [0001] The invention relates to sample preparation technology, in particular to a resin embedding device and an automatic liquid dosing method. Background technique [0002] The development of fMOST technology and the development of fluorescent labeling technology have promoted the reconstruction of neural circuits in the whole brain, so fluorescent labeling technology will be a very important research method in brain research. Therefore, in the process of sample processing, it is necessary to ensure that the experimental conditions of each step will not cause the quenching of fluorescence in the mouse brain, so as to meet the needs of three-dimensional high-resolution imaging of fluorescent samples. As we all know, fresh biological tissue is very soft and cannot meet the requirements of precision cutting. But because the system uses wide-field imaging, micron-level fluctuations on the sample surface can cause the image to be out of focus and blurred. In or...

AI Technical Summary

Problems solved by technology

[0005] Due to the long duration and cumbersome operation, it is difficult for the experimenter to ensure the accuracy of the solution ratio concentration, the accuracy of the soaking time, and the uniformity of the preparation conditions such as the experimental environment during the preparation of each sample, and the difference in the sample preparation conditions is It may lead to differences in sample imaging quality, and this will have a great impact on the variable control link in the experimental research process, which will further affect the authenticity and effectiveness of the experiment

In addition, continuous and intermittent operations in the preparation process will consume a lot of manpower and material resources, which also greatly reduces the overall experimental research efficiency of researchers.

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