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Method for detecting chemical components of kidney-tonifying and blood-activating formula

A detection method, a technique for nourishing the kidney and activating blood circulation, which is applied in the detection field of the chemical components of the prescription of nourishing the kidney and activating blood circulation, and can solve problems such as insufficient research

Active Publication Date: 2020-12-18
WANGJING HOSPITAL OF CHINA ACAD OF CHINESE MEDICAL SCI
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

[0004] However, there is still insufficient research on the medicinal mechanism or main components of the above-mentioned Bushen Huoxue prescription.

Method used

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  • Method for detecting chemical components of kidney-tonifying and blood-activating formula
  • Method for detecting chemical components of kidney-tonifying and blood-activating formula
  • Method for detecting chemical components of kidney-tonifying and blood-activating formula

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Embodiment 1

[0156] (1) Mix 225.2g of Eucommia ulmoides, 150.1g of psoraleae, 150.4g of Achyranthes bidentata, 181.0g of salvia miltiorrhiza, 151.1g of Clematis clematis and 135.2g of papaya, add 7.944L of water to soak for 12 hours, and heat and reflux at 95°C for 3 times , heating and refluxing each time is 1h, and the combined extracts are filtered, and the obtained filtrate is the water extract;

[0157] Concentrate the water extract under reduced pressure until the concentration of the traditional Chinese medicine is 1g / mL, then add ethanol with a volume fraction of 95% to the volume fraction of ethanol in the mixed solution to 70% for extraction, and then stand at 4°C for 24h at a speed of 4000r / min After centrifuging for 10 minutes, the obtained ethanol water supernatant was distilled under reduced pressure at 35°C until there was no alcohol smell, and the water extract was obtained;

[0158] Get 375mL of the water extract, add ethyl acetate for extraction, add ethyl acetate to the ...

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Abstract

The invention provides a method for detecting chemical components of a kidney-tonifying and blood-activating formula, and relates to the technical field of traditional Chinese medicine detection. Thedetection method provided by the invention comprises the following steps: sequentially carrying out water soaking, extraction, concentration, ethanol extraction, standing, centrifugal separation, removal of ethanol in supernatant, ethyl acetate extraction, n-butyl alcohol extraction of the obtained water phase, and concentration of the obtained water phase to obtain a to-be-detected liquid; and then carrying out UPLC-MS detection by adopting specific detection conditions, and comparing the obtained detection result with the standard chromatogram and the natural product database to obtain the chemical components in the to-be-detected liquid. The detection method provided by the invention can be used for qualitatively detecting 93 effective chemical components in the kidney-tonifying and blood-activating prescription at the same time, is simple to operate, and lays a foundation for the medicinal mechanism of the kidney-tonifying and blood-activating prescription and the treatment of lumbago by using traditional Chinese medicine monomers.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine detection, in particular to a method for detecting chemical components of a prescription for nourishing the kidney and activating blood. Background technique [0002] Low back pain is a common clinical disease and frequently-occurring disease. Its treatment is mainly based on surgery and non-steroidal anti-inflammatory drugs. Among them, surgical treatment is based on the risk of wound infection, nerve damage, and epidural hematoma, and non-steroidal anti-inflammatory drugs Side effects such as gastrointestinal bleeding can occur, and both treatment options are expensive. [0003] Traditional Chinese medicine treatment can solve the above-mentioned problems such as high surgical risk, large side effects and high price of western medicine. For example, Zhan Jiawen (Zhan Jiawen et al. Effect of Bushen Huoxue Prescription Containing Serum on Pressure Degeneration Model of Isolat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/34
CPCG01N30/02G01N30/06G01N30/34G01N2030/062
Inventor 朱立国
Owner WANGJING HOSPITAL OF CHINA ACAD OF CHINESE MEDICAL SCI
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