Ultralow-temperature preservation method for calluses of Oryza granulata

Abstract

The invention provides an ultralow-temperature preservation method for calluses of Oryza granulata. The ultralow-temperature preservation method comprises the steps that Oryza granulata seeds are selected, and the Oryza granulata seeds are disinfected, sterilized and inoculated to a culture medium to directly induce primary calluses; the Oryza granulata which cannot be directly induced into calluses, the seeds are disinfected and germinated, and when radicles grow to about 1cm, root segments are cut off to induce the calluses; after primary calluses of the Oryza granulata are obtained, two culture media are alternately used in a subculture multiplication process; and the propagated calluses are pre-cultured and collected, and are put into a culture dish with sterile filter paper for beingdried and directly cryopreserved. After unfreezing, the calluses are inoculated to the culture media for recovery culture, and differentiation culture is carried out after new calluses grow out untilgreen seedlings grow out. The ultralow-temperature preservation method for the calluses of the Oryza granulata is simple and feasible, the survival rate of the calluses is high after ultralow-temperature preservation, and an effective Oryza nivara resource preservation means is achieved.

Description

technical field [0001] The invention belongs to the technical field of plant cell engineering, and in particular relates to a cryopreservation method of granular wild rice callus. Background technique [0002] Rice is one of the three most important food crops in the world, and it is also the most important food crop in Asia. In the process of natural evolution, domestication and cultivation of cultivated rice for nearly 10,000 years, abundant rice genetic resources have been accumulated. However, with the rapidly deteriorating natural environment and unfavorable climatic conditions under the background of global industrialization, the promotion of mechanized and large-scale planting methods, and the yield-oriented breeding strategy have made the varieties of cultivated rice more and more simplistic, and the genetic basis of resources become narrow, genetic diversity is gradually lost, and the stress resistance genes in the original resources are gradually lost. As an impo...

AI Technical Summary

Problems solved by technology

However, the above-mentioned preservation methods are often complicated in steps, and have relatively high requirements for equipment (such as a program cooling instrument) or material requirements (such as small and tender materials such as stem tips and young ears, which are difficult to obtain materials), which is not conducive to the resources of granular wild rice. save