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A monoclonal antibody capable of competing with positive serum for binding to African swine fever virus b646l antigen and its application

A technology of African swine fever virus and monoclonal antibody, which is applied in the direction of anti-virus immunoglobulin, equipment, peptides, etc., can solve the problem of not being able to protect animals from virus attacks

Active Publication Date: 2021-04-23
CHINESE ACAD OF INSPECTION & QUARANTINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

African swine fever cannot cause a neutralizing reaction, but studies have shown that the protein encoded by the B646L gene has a conformational neutralizing epitope, which can induce the body to produce neutralizing antibodies, but unfortunately, the neutralizing antibodies cannot protect animals from virus attack

Method used

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  • A monoclonal antibody capable of competing with positive serum for binding to African swine fever virus b646l antigen and its application
  • A monoclonal antibody capable of competing with positive serum for binding to African swine fever virus b646l antigen and its application
  • A monoclonal antibody capable of competing with positive serum for binding to African swine fever virus b646l antigen and its application

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Experimental program
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Embodiment

[0084] 1. Materials

[0085] Fetal bovine serum, DMEM, HAT and HT are all products of Gibco in the United States; pcv3-positive serum, PRRS-positive serum, Seneca-positive serum, pseudorabies-positive serum, etc. are kept by our laboratory. Fifty inactivated ASF-positive sera and 200 ASFV-negative sera were donated by the National Veterinary Institute of Poland and the National Reference Laboratory for African Swine Fever of the Institute of Animal Health in Sardinia, Italy. Fifteen positive sera from inactivated pigs in the epidemic area and 100 negative sera from pigs imported from the United States in the non-infected area were provided by the National Reference Laboratory for African Swine Fever of the Institute of Animal Health in Sardinia, Italy. B646L Standard Antibody Detection Kit (Ingezim PPA Compac) was purchased from INGENASA, Jareza, Spain. Myeloma cell line SP2 / 0 was preserved in our laboratory, and Vero cells were preserved in our laboratory; Trizol was purchas...

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Abstract

The invention discloses an antigen-binding protein, an antibody or an active fragment capable of specifically competing with a positive serum to bind to an African swine fever virus B646L antigen, an African swine fever virus competition ELISA detection kit comprising the antigen-binding protein, an antibody or an active fragment, and Its preparation method and application. The African swine fever virus competition ELISA detection kit of the invention has high sensitivity and good specificity, and can be used for detecting the specific antibody of the African swine fever virus. The competition ELISA detection kit can specifically compete for the antibody that binds to the B646L antigen in the serum, and has the advantages of rapidity, simplicity, sensitivity and specificity in the clinical detection of the African swine fever antibody.

Description

technical field [0001] The invention belongs to the field of biology, and in particular relates to a monoclonal antibody capable of competing with positive serum for binding to the African swine fever virus B646L antigen and its application in a competitive ELISA detection method for detecting the African swine fever virus antibody. Background technique [0002] African swine fever (ASF) is an acute and highly fatal infectious disease of pigs caused by African swine fever virus (ASFV). ASF was discovered in Kenya in 1921, and has been present in sub-Saharan African countries since then, causing widespread prevalence in some countries in Western Europe and Latin America, causing serious economic losses. Since the first report in Shenyang in August 2018, my country has quickly spread to Shandong, Henan, Jiangsu, Hebei, Anhui, Beijing and other places. As of October 16, 2019, a total of 157 cases of African swine fever have been reported in my country, and a total of culled The...

Claims

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Application Information

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IPC IPC(8): C07K16/08G01N33/569G01N33/577G01N33/58
CPCC07K16/081C07K2317/35C07K2317/56G01N33/56983G01N33/577G01N33/581G01N2333/01G01N2469/10
Inventor 冯春燕王彩霞吴绍强林祥梅王勤刘晓飞
Owner CHINESE ACAD OF INSPECTION & QUARANTINE
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