Cell adherence microcarrier inoculation device and inoculation method
An inoculation device and microcarrier technology, applied in tissue cell/virus culture device, enzymology/microbiology device, method of supporting/immobilizing microorganisms, etc., can solve the problem of increasing vaccine production capacity and efficiency, prolonging virus amplification time , cell shedding and other problems, to reduce waiting time for filtration, prevent hypoxia, and increase the effect of filtration area
Pending Publication Date: 2020-12-25
BEIJING ZHONGYUAN HEJU ECONOMIC & TRADE
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Problems solved by technology
[0004] The invention provides a cell-attached microcarrier inoculation device, which can solve the problem of uniform microcarriers of VERO cells, provide uniformly attached seed cell microcarriers for large-scale bioreactors, and can also be applied to the pathological changes caused by cell inoculation virus , solve the problem of cells falling off from microcarriers, prolong the time of virus amplification and greatly increase the production capacity and efficiency of vaccines
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[0018] In order to facilitate the understanding of the present invention, the present invention will be described in more detail below in conjunction with the accompanying drawings and specific embodiments. Unless otherwise defined, all technical and scientific terms used in this specification have the same meaning as commonly understood by one of ordinary skill in the technical field of the invention. The terms used in the description of the present invention in this specification are only for the purpose of describing specific embodiments, and are not used to limit the present invention. The term "and / or" used in this specification includes any and all combinations of one or more of the associated listed items.
[0019] One embodiment of the present invention is that the present invention provides a cell-attached microcarrier inoculation device, which can solve the problem of uniform microcarriers for VERO cells, and provide uniform seed cell microcarriers for large-scale bi...
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Abstract
The invention discloses a cell adherence microcarrier inoculation device and an inoculation method. The method comprises the following steps that the inoculation device performs microcarrier cell inoculation, wherein the inoculation method specifically comprises the following steps of 1, sterilizing the inoculation device; 2, preheating the inoculation device; 3, preparation before inoculation; 4,filtering accumulated microcarrier cells; 5, filtering a perfusion process; 6, harvesting the inoculated microcarrier; and then obtaining the microcarrier / cell through the six steps. By the adoptionof the technical scheme, the empty ball rate in the inoculation process is eliminated, a double-pump and coaxial filter screen structure device is adopted, cells / microcarriers are evenly mixed throughdouble pumps and make contact in proportion, the cells and the microcarriers with the same concentration proportion are accumulated on the filter screen to form microcarrier accumulation buried pressure cells, and the cells are inoculated to the microcarriers in proportion.
Description
technical field [0001] The invention relates to the technical field of microcarrier inoculation process devices, in particular to a cell-adhered microcarrier inoculation device and an inoculation method. Background technique [0002] The new crown epidemic requires inactivated vaccines produced by a large number of adherent cells, and an enlarged microcarrier inoculation process device is urgently needed. At present, four inactivated vaccines against the new coronavirus SARS-CoV2 have been approved for clinical research in China. The clinical trial results have proved the safety and effectiveness of the new coronavirus inactivated vaccine. In order to adapt to future global use and reduce production costs, the production process needs to break through cell culture density and virus Production bottleneck. Domestic inactivated COVID-19 vaccines all use VERO-E6 cell culture to amplify the new coronavirus. VERO-E6 cells are adherent engineered cells, and growth requires adheren...
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IPC IPC(8): C12M3/04C12M1/32C12M1/12C12M1/00
CPCC12M25/02C12M29/04C12M33/04
Inventor 朱希灿
Owner BEIJING ZHONGYUAN HEJU ECONOMIC & TRADE