Compound microbial agent for preventing and treating soil-borne diseases of corn and preparation method and use of compound microbial agent
A technology of soil-borne diseases and compound bacteria, which is applied in the direction of botany equipment and methods, microbe-based methods, biochemical equipment and methods, etc., can solve the problems of general control effect, serious side effects, pollution, etc., and achieve good control effect , improve fertilizer efficiency, and improve the effect of soil
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0033] This embodiment provides a kind of preparation method of the compound microbial agent of preventing and treating corn soil-borne disease, it comprises the following steps:
[0034] S1. At a temperature of 37°C, place the preserved Bacillus subtilis on a slant medium for activating culture for 24 hours, and then place it in an environment of 120r / min and 37°C for 24 hours on a shaking table to obtain Bacillus subtilis mother liquor. Wherein, the slant culture medium adopted by Bacillus subtilis comprises the following components: 200 g of potatoes, 20 g of glucose, 20 g of agar, and 1000 mL of distilled water. In addition, Bacillus subtilis was obtained from the China Agricultural Microorganism Culture Collection Center, and its number is 19374.
[0035] S2. Sterilize the seed tank with high-temperature steam. After cooling, add the Bacillus subtilis liquid culture medium and the above-mentioned Bacillus subtilis mother liquor in the seed tank, and place it at 36° C. in...
Embodiment 2
[0040] This embodiment provides a kind of preparation method of the compound microbial agent of preventing and treating corn soil-borne disease, it comprises the following steps:
[0041] S1. At a temperature of 37°C, place the preserved Bacillus subtilis on a slant medium for activating culture for 24 hours, and then place it in an environment of 150r / min and 37°C for 24 hours on a shaking table to obtain Bacillus subtilis mother liquor. Wherein, the slant culture medium adopted by Bacillus subtilis comprises the following components: 200 g of potatoes, 20 g of glucose, 20 g of agar, and 1000 mL of distilled water.
[0042] S2. Sterilize the seed tank with high-temperature steam. After cooling, add the Bacillus subtilis liquid culture medium and the above-mentioned Bacillus subtilis mother liquor in the seed tank, and place it at 38°C in an aerobic environment for expanded cultivation to obtain 250 million Individual / mL Bacillus subtilis bacterial liquid of Bacillus subtilis...
Embodiment 3
[0047] This embodiment provides a kind of preparation method of the compound microbial agent of preventing and treating corn soil-borne disease, it comprises the following steps:
[0048] S1. At a temperature of 37°C, place the preserved Bacillus subtilis on a slant medium for activating culture for 24 hours, and then place it in an environment of 130r / min and 37°C for 24 hours on a shaking table to obtain Bacillus subtilis mother liquor. Wherein, the slant culture medium adopted by Bacillus subtilis comprises the following components: 200 g of potatoes, 20 g of glucose, 20 g of agar, and 1000 mL of distilled water.
[0049] S2. Carry out high-temperature steam sterilization to the seed tank. After cooling, add the Bacillus subtilis liquid culture medium and the above-mentioned Bacillus subtilis mother liquor in the seed tank, and place it in an aerobic environment at 37° C. for expanded cultivation to obtain 200 million Individual / mL Bacillus subtilis bacterial liquid of Bac...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More