Preparation method of spectral near-infrared electrochemical luminescence immunosensor

Abstract

The invention belongs to the field of analysis technical methods, and relates to preparation of a spectral near-infrared electrochemical luminescence (915nm) immunosensor. The preparation method mainly comprises the following steps that (1), methionine is used as a stabilizer to prepare a water-soluble Au-Ag bimetallic nanocluster capable of generating near-infrared electrochemical luminescence; (2), a gold-silver bimetallic nanocluster-marked secondary antibody (Au-AgAb2) is prepared; (3), a spectral near-infrared electrochemical luminescence sensor is prepared by taking the gold-silver bimetallic nano gold-silver cluster as a marker on the basis of a sandwich immunity principle; and (4), a working curve is drawn. The spectral electrochemical luminescence immunosensor is high in detectionsensitivity, and other antigen proteins do not interfere with the sensing detection of the target antigen.

Description

technical field

[0001] The invention belongs to the field of analytical techniques and methods, and relates to a preparation method of a spectrum-type near-infrared electrochemiluminescent immunosensor using gold-silver bimetallic nanomaterials as markers. Background technique

[0002] At present, most electrochemiluminescence (ECL) biochemical analysis and clinical diagnosis use ruthenium bipyridine whose maximum radiation wavelength is in the red light region (620 nm) as a marker, and it is realized by detecting the total intensity of ECL radiation. The development of new ECL biochemical analysis techniques and detection methods with different radiation bands is of great significance to promote the progress of ECL technology. For example: Chinese patent document CN104764737B discloses a light-intensity ECL immunoassay method with a radiation band located in the green light region (550nm) and with CdSe quantum dots as a marker; Chinese patent document CN102749452A discloses...

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