Method for detecting immune regulation function of neural stem cells

Abstract

The invention discloses a method for detecting an immune regulation function of neural stem cells. The method comprises the following steps of (1) conducting adherent culture of suspended neural spheres; (2) inactivating the neural stem cells; (3) marking hPBMC by CFSE; (4) conducting co-culture; (5) conducting lymphocyte subpopulation and proliferation detection; (6) conducting cytokine detection; (7) conducting data processing; and (8) conducting result analysis. According to the method, the hPBMC is utilized to simulate human immune response in vitro, and immunomodulatory effects of the neural stem cells with different sources, different generations, different culture modes and the like are studied, so that a basis is provided for process development of the neural stem cells. Accordingto the method, the nerve spheres subjected to suspension culture are digested into single cells, and then wall attachment is carried out, so that quantity control and inactivation of the single cellsare facilitated, and the proliferation condition of specific cell subsets can be visually detected by using a flow cytometry in combination with different lymphocyte subset antibodies by utilizing thecharacteristic that the CFSE is averagely distributed in a filial generation along with cell division. Various detection indexes are combined, so that operation steps are reduced, experimental materials are saved, and the cost is reduced.

Description

technical field [0001] The invention relates to a method for detecting the immune regulation function of neural stem cells, which belongs to the fields of stem cell technology research and immunology. Background technique [0002] Neural stem cell (NSC) refers to the existence in the nervous system, which has the potential to differentiate into neurons, astrocytes and oligodendrocytes, so that it can produce a large number of brain cell tissues and can carry out self-organization. Cell populations that are new and sufficient to provide a large number of brain tissue cells. Neural stem cell technology has developed rapidly in recent years, and it has great potential for clinical application in central nervous system injury diseases and other aspects. Not only that, neural stem cell therapy has obvious advantages, such as safety, sufficient sources of treatment materials, and a wide range of diseases to be treated. Many countries regard it as one of the important research and...

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