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Compound crystal of PARP15 protein and inhibitor Niraparib thereof, and preparation method for compound crystal

A technology of inhibitors and complexes, applied in the field of protein crystallization, can solve the problems of lack of protein research foundation, failure to obtain PARP15 protein crystallization, etc., and achieve the effect of simple conversion operation, short cycle and low cost

Inactive Publication Date: 2021-01-01
深圳晶蛋生物医药科技有限公司
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Problems solved by technology

[0006] The purpose of the present invention is to provide a method for successfully obtaining the complex crystal of PARP15 protein and its inhibitor Niraparib, so as to solve the problems in the prior art that the crystallization of PARP15 protein cannot be obtained and the basis for research on the protein is lacking

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  • Compound crystal of PARP15 protein and inhibitor Niraparib thereof, and preparation method for compound crystal
  • Compound crystal of PARP15 protein and inhibitor Niraparib thereof, and preparation method for compound crystal
  • Compound crystal of PARP15 protein and inhibitor Niraparib thereof, and preparation method for compound crystal

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[0031] The present invention will be described in detail below in conjunction with the accompanying drawings and specific embodiments, and the schematic embodiments and descriptions of the present invention are used to explain the present invention, but are not intended to limit the present invention.

[0032] 1. Construction of PARP15 protein expression plasmid

[0033] The gene encoding human PARP15 protein was cloned into the pET-28a vector to construct an expression plasmid. This work was entrusted to Shanghai Jierui Bioengineering Co., Ltd. The size of PARP15 protein is 26kDa, and the gene sequence encoding human PARP15 protein is shown in SEQ ID NO .1 shown.

[0034] SEQ ID NO.1:

[0035] 5'--3'

[0036] GGATCCATG AAT CTG CCG GAA CAT TGG ACC GAT ATG AAT CAT CAG CTG TTTTGC ATG GTG CAG CTG GAA CCG GGC CAG AGC GAA TAC AAT ACC ATT AAA GAT AAG TTTACC CGT ACC TGC AGC AGC TAT GCG ATT GAG AAA ATT GAA CGT ATT CAG AAT GCG TTTCTG TGG CAG AGC TAT CAG GTG AAA AAA CGT CAG ATG GAT...

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Abstract

The invention discloses a compound crystal of PARP15 protein and inhibitor Niraparib thereof, and a preparation method for the compound crystal. The preparation method comprises the following steps of: mixing the PARP15 protein with the inhibitor Niraparib thereof at a ratio; and carrying out crystallization on a mixture by a hanging drop method in a gaseous phase diffusion method to obtain the compound crystal of the PARP15 protein and the inhibitor Niraparib thereof. The preparation method has the advantages that the high-purity PARP15 protein is successfully obtained, in addition, the compound crystal of the PARP15 protein and the inhibitor Niraparib thereof is obtained for the first time, research on the PARP15 protein can be accelerated, and a molecular foundation is provided for designing and optimizing a targeted PARP15 protein medicine.

Description

technical field [0001] The invention relates to the technical field of protein crystallization, in particular to the PARP15 protein crystallization technology. Background technique [0002] PARP15 protein (polyadenosine diphosphate-ribose polymerase family member 15) is overexpressed in diffuse large B-cell lymphoma. Studies have reported that PARP15 protein has methylation activity. As tumor suppressor genes (TsGs) are often inactivated through somatic mutations and / or promoter methylation, PARP15 proteins have a suppressive effect on tumors. At present, there are few studies on PARP15 protein, and even less research on its biological function. However, the PARP family has a great influence on cancer, and some scholars believe that PARP15 protein is a candidate gene for cancer development and has the ability to regulate gene transcription. Furthermore, it has been reported to be involved in apoptosis. [0003] The three-dimensional structure of protein plays a very impo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/10C12N15/70C12R1/19
CPCC07K2299/00C12N9/1077C12N15/70C12Y204/0203
Inventor 李健熊兵
Owner 深圳晶蛋生物医药科技有限公司
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