Construction method of silkworm strain in small yellow croaker growth hormone bioreactor
A technology of bioreactor and growth hormone, which is applied in the direction of growth hormone, biochemical equipment and methods, chemical instruments and methods, etc., can solve the problems of unstable output and destruction of small yellow croaker resources, so as to promote the growth of small yellow croaker and improve the benefits of sericulture industry , Improve the effect of breeding efficiency
Active Publication Date: 2022-03-18
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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Problems solved by technology
However, due to the impact of overfishing and changes in the marine environment, the resources of small yellow croakers have been severely damaged, and the output is extremely unstable.
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[0037] (1) Cloning of small yellow croaker growth hormone gene and transferrin gene: using the whole genome sequencing information of small yellow croaker and transcriptome information of small yellow croaker, the growth hormone gene (GH) and transferrin gene (TF) of small yellow croaker were respectively obtained by homologous inspection method ) sequence, design the fusion gene of small yellow croaker growth hormone and transferrin; and optimize the fusion gene sequence according to the codon preference of silkworm. Before the fusion gene, add the IE2 promoter sequence, Kozak sequence and the coding sequence of the eukaryotic phosphate transport-related peptide in sequence, add the 6×His tag coding sequence and the SV40 sequence after the fusion gene, and finally add the coding sequence of the exogenous gene expression cassette to the two sides of the foreign gene expression cassette. The kpnⅠ and HindIII cloning sites were set at the end, and the synthetic insert was cloned ...
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The invention relates to a method for constructing a silkworm strain in a small yellow croaker growth hormone bioreactor, belonging to the technical field of silkworm transgenic genetic breeding. The method includes: (1) synthesizing the fusion gene of small yellow croaker growth hormone and transferrin; (2) obtaining piggy BAC transgenic plasmid pLX‑Red‑PcGH‑TF; (3) Transgenic plasmid introduced into silkworm fertilized eggs; (4) Obtaining G1 generation silkworm eggs; (5) Screening marker gene positive individuals; (6) Verifying the high efficiency of small yellow croaker growth hormone in silkworms expression; (7) identification of genetic stability; (8) establishment of small yellow croaker growth hormone bioreactor silkworm strain. In one aspect of the present invention, the small yellow croaker growth hormone is produced efficiently and at low cost, and can be further processed into a small yellow croaker functional additive for application in small yellow croaker breeding. On the other hand, it alleviates the development dilemma faced by sericulture production and improves the efficiency of sericulture industry.
Description
technical field [0001] The invention belongs to the technical field of silkworm transgenic genetic breeding, and in particular relates to a method for constructing a silkworm strain in a small yellow croaker growth hormone bioreactor. Background technique [0002] Since the reform and opening up, under the guidance of the development policy of "focusing on breeding", the development of aquaculture industry has made great achievements. The rapid development of the aquaculture industry has made outstanding contributions to solving the "difficulty in eating fish" of urban and rural residents, ensuring the supply of high-quality animal protein, reducing the utilization intensity of aquatic biological resources in natural waters, promoting the prosperity of the fishery industry, and making fishermen's lives rich. However, compared with the development requirements of the new era, the aquaculture industry still faces some difficulties and problems. In order to promote the green a...
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IPC IPC(8): C12N15/62C12N15/866C12N15/66A01K67/04
CPCC07K14/61C07K14/79C12N15/86C12N15/66C12N15/8509A01K67/04A01K67/0333C12N2800/22C12N2710/14043C07K2319/21A01K2207/15A01K2217/072A01K2227/70A01K2267/01
Inventor 翁宏飚牛宝龙沈卫锋
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES