Supercharge Your Innovation With Domain-Expert AI Agents!

A preparation method of hydrogel suitable for three-dimensional cell culture

A three-dimensional culture and hydrogel technology, applied in cell culture supports/coatings, biochemical equipment and methods, tissue culture, etc., can solve the problems of difficult to achieve precise control and poor mechanical properties of hydrogels, and achieve The gelling effect is stable, the growth is good, and the effect of improving stability

Active Publication Date: 2022-06-21
XI AN JIAOTONG UNIV
View PDF13 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional synthesis methods have developed hydrogels with excellent properties, such as contact lenses and some bioimplantation materials, etc., but it is difficult to achieve precise control during the synthesis reaction process, and the mechanical properties of the formed hydrogels are poor. One of the limiting factors of gels; in addition, the choice of materials also has a crucial impact on the living conditions and biological functions of cells in hydrogels, and many considerations are required, such as the plasticity of materials, biological Compatibility, safety and permeability, etc., but there are still many deficiencies in current research in this area

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A preparation method of hydrogel suitable for three-dimensional cell culture
  • A preparation method of hydrogel suitable for three-dimensional cell culture
  • A preparation method of hydrogel suitable for three-dimensional cell culture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] see Figure 1 to Figure 3 , a preparation method of hydrogel suitable for three-dimensional cell culture, the specific steps are:

[0056] Step 1: With the help of 3D printing, build a hydrogel forming mold, which is ready for use after disinfection;

[0057] Step 2: Prepare the required solutions for the hydrogel respectively, taking the preparation of 1ml of the hydrogel as an example:

[0058] 1) Preparation of NaOH solution: Take 0.96g of NaOH powder, put it into a clean centrifuge tube, add 10ml of double-distilled water, fully dissolve and mix, and prepare a solution with a concentration of 2.4mmol / L; in the ultra-clean bench, use 0.22μm Filter and sterilize the filter; put the sterile solution into a clean centrifuge tube, seal it, and store it at 4°C for future use;

[0059] 2) Preparation of calcium ion cross-linking solution (CaCO 3 -GDL): take CaCO 3 - 0.87g of GDL powder, put it into a clean centrifuge tube, add 10ml of double-distilled water, fully diss...

Embodiment 2

[0084] A preparation method of a hydrogel suitable for three-dimensional cell culture, the specific steps are:

[0085] Step 1: With the help of 3D printing, build a mold for hydrogel molding, and use it after sterilization;

[0086] Step 2: Prepare the required solutions for the hydrogel respectively, taking the preparation of 1ml of the hydrogel as an example:

[0087] 1) Preparation of NaOH solution: Take 1.04g of NaOH powder, put it into a clean centrifuge tube, add 10ml of double-distilled water, fully dissolve and mix, and prepare a solution with a concentration of 2.6mmol / L; in the ultra-clean bench, use 0.22μm Filter and sterilize the filter; put the sterile solution into a clean centrifuge tube, seal it, and store it at 4°C for future use;

[0088] 2) Preparation of calcium ion cross-linking solution (CaCO 3 -GDL): take CaCO 3 - 1.22g of GDL powder, put into a clean centrifuge tube, add 10ml of double distilled water, fully dissolve and mix to make a solution with ...

Embodiment 3

[0113] A preparation method of a hydrogel suitable for three-dimensional cell culture, the specific steps are:

[0114] Step 1: With the help of 3D printing, build a hydrogel forming mold, which is ready for use after disinfection;

[0115] Step 2: Prepare the required solutions for the hydrogel respectively, taking the preparation of 1ml of the hydrogel as an example:

[0116] 1) Preparation of NaOH solution: Take 1.12g of NaOH powder, put it into a clean centrifuge tube, add 10ml of double-distilled water, fully dissolve and mix to make a concentration of 2.8mmol / L; in the ultra-clean bench, use a 0.22μm filter Filter and sterilize; put the sterile solution in a clean centrifuge tube, seal it, and store it at 4°C for later use;

[0117] 2) Preparation of calcium ion cross-linking solution (CaCO 3 -GDL): take CaCO 3 - 1.39g of GDL powder, put into a clean centrifuge tube, add 10ml of double distilled water, fully dissolve and mix to make a solution with a concentration of ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

A hydrogel preparation method suitable for three-dimensional cell culture. The mold for constructing hydrogel molding is produced by 3D printing, which is sterilized and ready for use; collagen-NaOH solution, calcium ion cross-linking solution, and sodium alginate solution are mixed according to a certain ratio. , modified Eagle medium (DMEM medium, Dulbecco's Modified Eagle Medium) and cell suspension, after rapid shaking and mixing, a clear and translucent colloidal solution was obtained, which was transferred to a mold and incubated in a 37°C constant temperature incubator for 1h Post-molding; the invention has the advantages of good biocompatibility, safety and degradability, simple operation steps and stable gelling effect.

Description

technical field [0001] The invention belongs to the technical field of biological materials, and in particular relates to a hydrogel preparation method suitable for three-dimensional cell culture. Background technique [0002] Hydrogels are water-swellable polymeric materials that maintain unique three-dimensional structures and have been widely used in tissue engineering. Traditional synthesis methods have developed hydrogels with excellent properties, such as contact lenses and some biological implant materials, but it is difficult to achieve precise control during the synthesis reaction, and the mechanical properties of the formed hydrogels are poor. A major limiting factor for gels; in addition, the choice of materials also has a crucial impact on the living state and biological functions of cells in hydrogels, requiring many considerations, such as material plasticity, biological Compatibility, security and permeability, etc., and there are still many deficiencies in t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/38A61L27/02A61L27/20A61L27/24A61L27/52A61L27/58C12N5/00
CPCA61L27/24A61L27/20A61L27/02A61L27/38A61L27/52A61L27/58C12N5/0062C12N2533/54C12N2533/74C12N2513/00C08L89/00C08L5/04
Inventor 邹蕊吴诗阳牛林郑丹青亢雪萍王译婕赵舒扬
Owner XI AN JIAOTONG UNIV
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com