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Application of entomopathogenic fungus, method for controlling pests and insecticide

A technology of pathogenic fungi and pesticides, applied in the field of microorganisms, can solve problems such as reduced yield, weakened growth of damaged plants, and impact on the ecological environment

Active Publication Date: 2021-05-07
QIQIHAR BRANCH OF HEILONGJIANG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition to eating leaves and absorbing corn juice, corn aphid (Rhopalosiphum maidis) will also secrete a large amount of honeydew, which will affect the photosynthesis of crops, and the growth of the damaged plants will become weaker, and the yield will decrease. At present, the control of corn aphids is mainly based on chemical control. Excessive spraying of pesticides will affect the ecological environment and kill some beneficial microorganisms

Method used

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  • Application of entomopathogenic fungus, method for controlling pests and insecticide
  • Application of entomopathogenic fungus, method for controlling pests and insecticide
  • Application of entomopathogenic fungus, method for controlling pests and insecticide

Examples

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Embodiment 1

[0029] 1. Isolation method of entomopathogenic fungi

[0030] An entomopathogenic fungus (SGSF043) was isolated from understory litter in Daxing'an Mountains. The undergrowth litter samples collected in the Daxing'an Mountains were dried and crushed into particles, and filtered with a filter to obtain particles with a particle size of 100-200 μm in the litter samples. Using the particle plate dilution method, the granular sample was mixed with sterile water at a mass ratio of 1:100 to prepare a particle suspension. Then use a pipette gun to draw 50 μL, 100 μL, 150 μL, 200 μL and 250 μL and apply them to the PDA medium plate respectively, spread it with a coating rod until the particles are evenly dispersed, place it at room temperature for cultivation, and observe it every 12 hours until the fungal colonies grow out , the colonies were picked to PDA plates for purification. After microscopic observation of morphology, ITS and TEF sequence amplification and sequencing, an ent...

Embodiment 2

[0056] Embodiment 2. The method for anti-pest activity assay

[0057] One, the method for preparing the spore suspension of entomopathogenic fungus

[0058] First prepare the spore suspension, inoculate the strain on the PDA medium and cultivate for 14 days, add 5 mL of sterilized 0.05% Tween 80 aqueous solution on the plate, and scrape the surface spores. After filtering out excess mycelium and agar with sterilized cotton, use a hemocytometer to count and adjust the spore suspension to a final concentration of 1×10 8 individual / mL.

[0059] Two, the method that the spore suspension of entomopathogenic fungi treats pests

[0060] Soak the two-spotted long tarsus beetle (adult) in 1×10 8 Individuals / mL concentration of the spore suspension for 5s, and then transferred to a Erlenmeyer flask filled with corn kernels for observation, and 35 adults were inoculated for each treatment. Soak mung bean weevils (adults) in 1×10 8 spores / mL (spores spore number) concentration of the...

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Abstract

The invention provides an application of entomopathogenic fungi, a method for controlling pests and an insecticide, belonging to the field of microorganisms. In order to avoid the impact of chemical pest control methods on the ecological environment, the invention provides an entomopathogen numbered SGSF043 In the application of fungi, the SGSF043 strain has a higher lethality rate to the two-spotted leaf beetle. The cumulative corrected mortality rate reached 91.55% on the 8th day of the inoculation treatment, and the dead insect rate was above 90%. In 3 days, the lethality rate reached 100%, and the final dead insect rate was more than 70%. The SGSF043 bacterial strain corrected the mortality rate to reach more than 50% when infecting mung bean elephant on the 8th day, indicating that the SGSF043 bacterial strain had good insect-resistant activity and could invade It infects a variety of pests and has a wide range of control. It belongs to non-host specialized entomopathogenic fungi and has good biological control potential for pests. It can be used as a biological control strain.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to an application of entomopathogenic fungi, a method for controlling pests and an insecticide. Background technique [0002] As a common biological control fungus, entomopathogenic fungi have been widely used in pest control, and there have been many successful cases. Scarab entomopathogenic fungi (Metarhizium anisoliae), yellow-green entomopathogenic fungi (M.flavoviride) and Robert's insects Pathogenic fungus (M.robertsii) and other strains are effective against grape black weevil (Otiorhynchus sulcatus), cricket (Anabrus simplex), diamondback moth (Plutella xylostella), gray planthopper (Laodelphax striatellus), etc. Coleoptera, Orthoptera, Lepidoptera, etc. Insects of the order and Hemiptera are all infective. Monolepta hieroglyphica is an omnivorous pest with a wide range of food. There are some reports on chemical control, but no reports on biological control. In a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/30A01P7/04
CPCA01N63/30
Inventor 郑旭张超杨慧莹赵秀梅徐利剑高思禹张李香宋福强
Owner QIQIHAR BRANCH OF HEILONGJIANG ACADEMY OF AGRI SCI