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Application of ASTE1 in detection of esophageal squamous cell carcinoma

A technology for esophageal squamous cell carcinoma and a detection kit, which is applied in the fields of biomedicine and molecular biology, and can solve the problems of no reports on the biological functions of esophageal squamous cell carcinoma

Active Publication Date: 2021-01-12
THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no primary disease-associated mutation of ASTE1, but it has been reported in recent years that ASTE1 is closely related to the occurrence of colorectal cancer, but its biological function in esophageal squamous cell carcinoma has not been reported yet

Method used

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  • Application of ASTE1 in detection of esophageal squamous cell carcinoma
  • Application of ASTE1 in detection of esophageal squamous cell carcinoma
  • Application of ASTE1 in detection of esophageal squamous cell carcinoma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Establishment of differential gene expression profiles between asymptomatic population and esophageal squamous cell carcinoma tissues using gene chips

[0024] 1. Experimental samples

[0025] After standardizing the clinical diagnosis, treatment, pathology and follow-up information database of 500,000 cases of esophageal cancer and cardia cancer, 10 cases of esophageal squamous cell carcinoma samples were finally selected, and the inclusion criteria were: ① underwent radical surgery for esophageal squamous cell carcinoma; ③ complete follow-up data; ④ preoperative serum and postoperative tissue samples; ⑤ all samples from the same municipal hospital. Among the 10 samples of esophageal squamous cell carcinoma, all patients were diagnosed between January 2019 and December 2019. There were 6 males and 4 females; the average age at diagnosis was 60.5±4.3 years old; the age range was 55-75 years old; the medullary type 3 cases, 2 cases of mushroom type, 3 cases of...

Embodiment 2

[0035] Application of the primer sequence of embodiment 2 ASTE1 in real-time quantitative PCR kit

[0036] 1. Experimental samples

[0037]After standardizing the clinical diagnosis, treatment, pathology and follow-up information database of 500,000 cases of esophageal cancer and cardia cancer, 10 cases of esophageal squamous cell carcinoma samples were finally selected, and the inclusion criteria were: ① underwent radical surgery for esophageal squamous cell carcinoma; ③ complete follow-up data; ④ preoperative serum and postoperative tissue samples; ⑤ all samples from the same municipal hospital. Among the 10 samples of esophageal squamous cell carcinoma, all patients were diagnosed between January 2019 and December 2019. There were 6 males and 4 females; the average age at diagnosis was 60.5±4.3 years old; the age range was 55-75 years old; the medullary type 3 cases, 2 cases of mushroom type, 3 cases of ulcer type, 2 cases of constriction type. 5 cases of normal esophagea...

Embodiment 3

[0049] Example 3 Application of ASTE1 protein in ELISA detection kit

[0050] After standardizing the clinical diagnosis, treatment, pathology and follow-up information database of 500,000 cases of esophageal cancer and cardia cancer, 1,000 cases of esophageal squamous cell carcinoma samples were finally selected, and the inclusion criteria were: ① underwent radical surgery for esophageal squamous cell carcinoma; ③ complete follow-up data; ④ preoperative serum and postoperative tissue samples; ⑤ all samples from the same municipal hospital. Among the 1000 samples of esophageal squamous cell carcinoma, all patients were diagnosed between January 2018 and December 2019, 546 males and 454 females; the average diagnosis age was 60.5±7.3 years old; the age range was 52-86 years old; the stage of esophageal cancer 196 cases of stage I, 514 cases of stage II, 222 cases of stage III, and 68 cases of stage IV. Esophageal squamous cell carcinoma 100 cases of normal esophageal tissue sa...

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Abstract

The invention belongs to the technical field of biomedicine and molecular biology, and relates to application of ASTE1 in preparation of an esophageal squamous cell carcinoma detection kit. A differential gene expression profile between an asymptomatic population and esophageal squamous cell carcinoma tissues is established by using a gene chip kit, the level of ASTE1mRNA in the asymptomatic population and the esophageal squamous cell carcinoma tissues is measured by using a real-time quantitative PCR technology, and the content of ASTE1 in serum samples of the asymptomatic population and theesophageal squamous cell carcinoma patients is detected by using a sandwich ELISA method. Results prove that the ASTE1 can be used as a novel serum marker for esophageal squamous cell carcinoma, can be used for further preparing an ELISA diagnostic kit for detecting esophageal squamous cell carcinoma, and provides important reference information for diagnosis of preoperative or early squamous cellcarcinoma.

Description

technical field [0001] The invention belongs to the technical fields of biomedicine and molecular biology, and specifically relates to the application of ASTE1 in detecting esophageal squamous cell carcinoma. Background technique [0002] Of the approximately 500,000 new cases of esophageal cancer each year in the world, more than half occur in China. Linzhou City, Henan Province is China, and it is also the region with the highest incidence and mortality of esophageal cancer in the world, which is 100 times higher than that in Western countries Currently, esophageal cancer is still the leading cause of cancer-related death in this region. Due to the lack of specific symptoms in early stage patients, about 90% of them are in the middle and late stage when they first visit the doctor, and the 5-year survival rate of the middle and late stage patients is only about 10%, so the prognosis of patients with esophageal cancer is poor. However, the 5-year survival rate of patients ...

Claims

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Application Information

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IPC IPC(8): G01N33/574G01N33/564G01N33/58G01N33/543C12Q1/6851C12Q1/6886
CPCC12Q1/6851C12Q1/6886C12Q2600/158G01N33/543G01N33/564G01N33/57407G01N33/57484G01N33/581C12Q2531/113C12Q2545/101C12Q2561/113C12Q2563/107
Inventor 王立东雷玲玲宋昕赵学科徐瑞华范宗民伊力亚尔·夏合丁毛伟敏王献增
Owner THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV