Zebra fish cerebral arterial thrombosis model, construction method and application

A technology of ischemic stroke and construction method, applied in the field of biomedicine, can solve the problems of infarction, loose brain tissue, etc.

Active Publication Date: 2021-01-22
JINGGANGSHAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

After further research, it was found that this method mainly caused loose and infarcted parts of the brain tissue.

Method used

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  • Zebra fish cerebral arterial thrombosis model, construction method and application

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Embodiment 1

[0022] A method for constructing a zebrafish ischemic stroke model, selecting wild-type adult zebrafish of the normally developing AB strain, injecting β-amyloid protein solution at a concentration of 0.01 μg / ml in the head of the normally developing adult zebrafish, injecting The dose was 1 μL / kg body weight. Immediately after injection, the zebrafish were placed in 25°C normal saline for 20 minutes, and then transferred to 25°C feeding solution containing lead nitrate for 4 days; the concentration of lead nitrate in the feeding solution was 1mg / 100ml.

Embodiment 2

[0024] A method for constructing a zebrafish ischemic stroke model, selecting wild-type adult zebrafish of the normally developing AB strain, injecting β-amyloid protein solution at a concentration of 0.01 μg / ml in the head of the normally developing adult zebrafish, injecting The dose was 1 μL / kg body weight. Immediately after injection, the zebrafish were placed in 25°C normal saline for 40 minutes, and then transferred to 25°C feeding solution containing lanthanum nitrate for 4 days; the concentration of lanthanum nitrate in the feeding solution was 1mg / 100ml.

Embodiment 3

[0026] A method for constructing a zebrafish ischemic stroke model, selecting wild-type adult zebrafish of the normally developing AB strain, injecting β-amyloid protein solution at a concentration of 0.01 μg / ml in the head of the normally developing adult zebrafish, injecting The dose was 1 μL / kg body weight. Immediately after injection, the zebrafish were placed in 25°C normal saline for 20 minutes, and then transferred to 25°C feeding solution containing lead acetate for 5 days; the concentration of lead acetate in the feeding solution was 2mg / 100ml.

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Abstract

The invention belongs to the technical field of biomedicines, and particularly relates to a zebra fish cerebral arterial thrombosis model, a construction method and application. Beta-amyloid protein liquid with the concentration of 0.01-0.05 mu g/ml is injected into the head of a normally developed adult zebra fish according to the injection amount of 1 mu L/kg of body weight, the zebra fish is placed into normal saline for 20-40 min after injection, and then the zebra fish is transferred into feeding liquid containing an inducer for feeding for 4-7 d; and the inducer is a soluble lanthanum compound or a soluble lead compound. The cerebral arterial thrombosis model of cerebellar obstruction is successfully constructed, and a foundation is laid for research and development of cognitive function nerve injury related drugs.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a zebrafish ischemic stroke model, a construction method and application. Background technique [0002] Stroke is a vascular disease that transports blood to the brain. As the name implies, ischemic stroke is a disorder of blood supply to local brain tissue, resulting in damage or loss of brain nerve function. After a stroke, there may be serious consequences affecting life activities and motor dysfunction, resulting in the inability of the human body to take care of itself, so it is a difficult problem that the medical community pays attention to. Stroke may occur in different areas of the human brain, which will lead to damage to different physiological functions. Currently, there are very limited types of drugs for the treatment of stroke, such as butylphthalide. Although they have certain clinical curative effects, they also have liver toxicity and kidney dama...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/10
CPCA01K61/10A01K2267/0375A01K2227/40A01K2207/10A01K2207/20Y02A40/81
Inventor 曹子岗乐青
Owner JINGGANGSHAN UNIVERSITY
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