High-precision ultra-thin isopickness slicer for fresh tissue sectioning

Abstract

The invention discloses a high-precision ultra-thin equal-thickness slicer for fresh tissue slicing, which includes a mounting base, the mounting base includes a mounting box body and a mounting bottom plate, and the mounting box body is provided with slider connectors, A linear motor and an encoder; a guide rail is provided on the top surface of the slider connector, and the guide rail includes a slider, and a groove is provided on the top of the slider, and a bearing member that slides back and forth is arranged in the groove; The top of the carrier is fixed to the inner wall of the top of the installation box; the top surface above the tool installation wall of the slider connector is provided with an encoder boss, the linear grid is fixed on the boss, and the top of the reading head is connected to the installation box The top wall of the body is fixed; the cutter adjustment frame is fixed on the cutter installation wall of the slider connector. The combination of the linear motor drive and the encoder feedback mode of the slicer of the present invention forms a cutter vibrating device with simple structure and stable performance, which can control the vibration frequency and amplitude and meet the slicing requirements of different samples.

Description

technical field [0001] The invention relates to biological research and medical instruments, in particular to a high-precision ultrathin equal-thickness slicer for fresh tissue slices. Background technique [0002] Microtome is an off-the-shelf technology that is widely used in biological research and medical imaging to prepare thin sections of fresh tissue samples for further staining and microscopic imaging. Usually, living tissue and low-glue agarose or paraffin-embedded fresh tissue are too soft to obtain thin sections with hard cutters, and the sections are of equal thickness. The main solution at present is to provide a vibrating microtome, in which the blade vibrates back and forth along the direction of the blade (Y direction), and at the same time the blade moves relative to the sample along the X direction to complete the ultra-thin sectioning process. Slices can be up to ten microns or even several microns thick, and the equal thickness error is less than 1 micro...

AI Technical Summary

Problems solved by technology

[0003] Chinese patent CN101500767 discloses a vibrating microtome based on the structure of eccentric wheel and guide rail. The motor drives the eccentric wheel to rotate, thereby driving the blade to reciprocate and vibrate along the guide rail to achieve vibratory sectioning of the sample tissue, but the vibration amplitude of the blade cannot be adjusted. , and the guide rail is prone to wear and tear during long-term use, resulting in serious Z-direction errors

[0004] Chinese patent CN104729874 describes a high-precision vibrating slicer, which is mainly realized based on electromagnetic force drive and spring steel sheet. The electromagnetic force drives the blade to resonate with the spring steel sheet to achieve reciprocating vibration. Although there is a mechanism for adjusting the Z-direction error , but the spring steel sheet not only provides the stiffness in the vibration direction, but also provides the overall support of the blade. During the vibration process, the Z-direction vibration cannot be controlled, resulting in serious Z-direction errors.

[0005] U.S. Patent US6041686 describes a vibrating microtome based on the eccentric wheel and guide rail structure. Through the specially designed eccentric wheel structure, the vibration amplitude of the blade can be adjusted, but the adjustment range is limited, and only a few fixed values ​​can be selected. At the same time, there is no control of Z To the error mechanism, and the guide rail wears out during long-term use, which will cause serious Z-direction error

[0006] U.S. Patent US6651538 describes a vibrating microtome based on a leaf spring structure and driven by electromagnetic force, which avoids the wear of the guide rail during long-term use, but uses the interaction force between the electromagnet and the permanent magnet as the driving force , it is difficult to ensure that the direction of the driving force is consistent with the vibration direction of the blade, and there is no mechanism to control the Z-direction error, which cannot reduce the Z-direction error during the blade vibration process, which will affect the slicing effect of the sample tissue

[0007] To sum up, the drive mode and structural form of the existing vibrating microtome are unreasonable, and even lack a mechanism for fine-tuning the Z direction of the blade, which cannot ensure that the direction of the blade is consistent with the vibration direction, and Z-direction errors are prone to occur, resulting in ultra-thin slices. Unable to achieve, let alone the equal thickness requirements of ultra-thin slices

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