Melanin hair dye and use method thereof
A hair dye and melanin technology, applied in hair care, pharmaceutical formulations, cosmetic preparations, etc., can solve the problems of hair color change, unstable color of hair dye, fading, etc. The effect of short hair dyeing time
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Embodiment 1
[0026] A melanin hair dye, the preparation steps of which are as follows:
[0027] (1) Melanocyte culture: normal isolated primary human melanocytes (P3) were cultured with M254 medium, and when the cell confluence reached 90%, the cells were digested (0.25% trypsin), centrifuged (800rpm / min, After 5min), the cells were suspended in PBS, and then the suspension was irradiated with a UVB dose of 100mJ / cm2. After centrifugation, the culture was continued at a ratio of 1:2 for 24h, and then placed in a -80°C refrigerator for later use.
[0028] (2) Obtaining melanin granules: freeze-thaw the melanocytes obtained in step (1) repeatedly (3-4 times), collect the liquid, filter, acidify with citric acid, and centrifuge to obtain melanin granules as precipitates. (3) Preparation of melanin hair dye:
[0029] This hair dye includes three dosage forms, agent A: based on 100 parts by weight, add 5 parts of L-cysteine, 0.6 part of ascorbic acid (VC), 1 part of hydroxyethyl cellulose, 0.3...
Embodiment 2
[0034] A melanin hair dye, the preparation steps of which are as follows:
[0035] (1) Melanocyte culture: The normal isolated primary human melanocytes (P4) were cultured with M254 medium. When the cell confluence reached 90%, the cells were digested (0.25% trypsin), centrifuged (800rpm / min, After 5min), the cells were suspended in PBS, and then the suspension was irradiated with a UVB dose of 150mJ / cm2. After centrifugation, the culture was continued at a ratio of 1:2 for 36h, and then placed in a -80°C refrigerator for use.
[0036] (2) Obtaining melanin granules: The melanocytes obtained in step (1) were repeatedly frozen and thawed 4 times, the liquid was collected, filtered, acidified with citric acid, and centrifuged to obtain melanin granules as precipitates.
[0037] (3) Preparation of melanin hair dye:
[0038] This hair dye comprises three dosage forms, A agent: add 4 parts of L-cysteine, 0.8 part of ascorbic acid (VC), 0.8 part of guar gum, 2 parts of preservative...
Embodiment 3
[0043] A melanin hair dye, the preparation steps of which are as follows:
[0044] (1) Melanocyte culture: The normal isolated primary human melanocytes (p4) were cultured with M254 medium, and when the cell confluence reached 90%, the cells were digested (0.25% trypsin), centrifuged (800rpm / min, After 5min), the cells were suspended in PBS, and then the suspension was irradiated with a UVB dose of 150mJ / cm2. After centrifugation, the culture was continued at a ratio of 1:3 for 48h, and then placed in a -80°C refrigerator for use.
[0045] (2) Obtaining melanin granules: freeze-thaw the melanocytes obtained in step (1) three times, collect the liquid, filter, acidify with citric acid, and centrifuge to obtain melanin granules as precipitates.
[0046] (3) Preparation of melanin hair dye:
[0047] This hair dye includes three dosage forms, agent A: 4 parts of glutathione, 0.4 part of ascorbic acid (VC), 0.8 part of carboxymethylcellulose sodium, 1.5 parts of preservative ( ph...
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