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Application of pueraria flower spleen-strengthening-relieving formula drug-containing serum in cancer cells

A cancer cell and liver cancer cell technology, applied in the field of medical invention, can solve the problem of low sensitivity of liver cancer to radiotherapy and chemotherapy, and achieve the effect of inhibiting tumor invasion and metastasis and reducing cell migration rate

Pending Publication Date: 2021-01-26
贵州中医药大学
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, surgery is still the main treatment for liver cancer, but metastasis and invasion are the main causes of postoperative recurrence and death in patients with liver cancer. In addition, liver cancer is less sensitive to radiotherapy and chemotherapy, so it is very realistic to find suitable and effective treatment methods and drugs significance [5-8]

Method used

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  • Application of pueraria flower spleen-strengthening-relieving formula drug-containing serum in cancer cells
  • Application of pueraria flower spleen-strengthening-relieving formula drug-containing serum in cancer cells
  • Application of pueraria flower spleen-strengthening-relieving formula drug-containing serum in cancer cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Application of medicated serum of Gehua Jiefeng recipe in the migration and invasion of HepG2 liver cancer cells and the expression of E-cadherin and N-cadherin proteins.

[0031] 1 Materials and methods

[0032] 1.1 Experimental materials

[0033] 1.1.1 Experimental animals and cells

[0034] SD rats, half male and half male, SPF grade, weighing 180-200 g, 40 were purchased from Changsha Tianqin Biotechnology Co., Ltd., certificate number: SCXK (Xiang) 2014-0011. The human liver cancer HepG2 cell line was purchased from the Shanghai Cell Bank of the Chinese Academy of Sciences, numbered SCSP-510, and was routinely cultured, passaged, and frozen in strict accordance with the instructions, and the experiment was performed when it was in the logarithmic growth phase.

[0035] 1.1.2 Experimental drugs

[0036] Gehua Jiezhen prescription consists of 0.9 grams of lotus green peel, 1.5 grams of woody fragrance, 4.5 grams of orange peel, 4.5 grams of ginseng, 4.5 grams of p...

Embodiment 2

[0069] Application of medicated serum of Gehua Jiejiu formula on the proliferation ability and apoptosis of HepG2 liver cancer cells.

[0070] 1 Materials and methods

[0071] 1.1 Experimental materials

[0072] 1.1.1 Experimental animals and cells

[0073] SD rats, male and female, SPF grade, weighing 180-200g, 20 were purchased from Changsha Tianqin Biotechnology Co., Ltd., certificate number: SCXK (Xiang) 2014-0011. The human liver cancer HepG2 cell line was purchased from the Shanghai Cell Bank of the Chinese Academy of Sciences, numbered SCSP-510, and was routinely cultured, passaged, and frozen in strict accordance with the cell instructions.

[0074] 1.1.2 Experimental drugs

[0075] Gehua Jiezhen prescription consists of 0.9 grams of lotus green peel, 1.5 grams of woody fragrance, 4.5 grams of orange peel, 4.5 grams of ginseng, 4.5 grams of polyporus, 4.5 grams of white poria cocos, 6 grams of divine music, 6 grams of Alisma, 6 grams of dried ginger, and Atractylode...

Embodiment 3

[0106] The drug-containing serum of the Gehua Jiezhen prescription is taken as a raw material drug, 1 / 30 of starch is added, and granulated to obtain granules.

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Abstract

The invention discloses application of pueraria flower spleen-strengthening-relieving formula drug-containing serum in cancer cells. By preparing the pueraria flower spleen-strengthening-relieving formula drug-containing serum, observing the influence of the drug-containing serum with different concentrations on the invasion, transfer and epithelial-mesenchymal transition of HepG2 hepatoma carcinoma cells and observing the influence of the drug-containing serum with different concentrations on the proliferation and apoptosis of the HepG2 hepatoma carcinoma cells, the pueraria flower spleen-strengthening-relieving formula drug-containing serum is proved to have the effects of inhibiting the proliferation of the HepG2 hepatoma carcinoma cells, promoting the apoptosis of the HepG2 hepatoma carcinoma cells and the like, and theoretical and data support is provided for the clinical anti-hepatoma metastasis application of the formula.

Description

technical field [0001] The invention relates to the field of medical inventions, in particular to the application of a drug-containing serum of Gehua Jiejiu formula in cancer cells. Background technique [0002] Primary liver cancer is a malignant tumor with a high incidence in my country. According to reports, new patients in my country account for about half of the total number of patients in the world every year, and the trend is increasing year by year. [1 2] . However, because the onset of liver cancer is relatively hidden, most patients have entered the middle and late stages when they are discovered, the treatment effect is poor, and there are many complications, so the prognosis is extremely poor. However, the occurrence and development of liver cancer is a multi-stage and multi-process progression. From the initial hepatitis and cirrhosis to the final diagnosis of liver cancer, there will be a long period of precancerous lesions in the liver. [3 4] . Therefore,...

Claims

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Application Information

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IPC IPC(8): A61K35/16A61K36/9068A61P35/00A61P35/04
CPCA61K35/16A61K36/62A61K36/285A61K36/752A61K36/258A61K36/07A61K36/076A61K36/00A61K36/884A61K36/9068A61K36/284A61K36/9064A61K36/488A61P35/00A61P35/04
Inventor 李军杨柱唐东昕何金秀
Owner 贵州中医药大学
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