Stirrer for plant cell culture device

Abstract

The invention discloses a stirrer for a plant cell culture device. The stirrer structurally comprises a motor, a stirring mechanism, a fixation plate, a controller and a lifting plate, wherein the stirring mechanism is provided with a fixation groove, a stirring device, a placing tank, a fixer and a blocking cover. Liquid generates inward force under the action of rotating force, and the effect ofreducing the rotating force is achieved; the law that liquid rotates along the inner wall is destroyed through staggering of circulating grooves, so that the liquid circulates back and forth, the pressure of rotation on the liquid is reduced, and cells in the liquid are prevented from being broken; the liquid moves upwards along drainage grooves in the inner sides of sliding plates under the action of rotating force, and therefore the horizontal position of the liquid is changed; and according to mechanical principles, liquid is expanding outwards, and moves upwards along inclined sealing grooves under the rotating force to compress sealing grooves, so that stress blocks are stressed, liquid on the inner sides of rubber plates fluctuates, the fluctuation applies upward pressure under therotating force, then the liquid is driven to be stirred upwards, and the mixing degree of the liquid is improved.

Description

technical field [0001] The invention relates to the field of animal and plant cell culture devices, in particular to a stirrer for plant cell culture devices. Background technique [0002] Animal and plant cell culture uses cell culture medium for stirring to provide nutrients for plant cells, so that they can get nutrients in the culture medium. Through stirring, the cells can be fully and evenly contacted with the culture medium, so as to achieve a better culture effect, and to reduce stirring The shear force of the impeller baffle agitator with a larger cut is used, but the agitator with the baffle structure will generate a large centrifugal force when the stirring speed is too fast, so that the liquid rotates along the inner wall of the container, which is easy to make the plant cell chloroplast Pressure is generated, which causes the rupture of the cell membrane, resulting in the loss of plant cell activity, and because the inner wall of the container is relatively smoo...

AI Technical Summary

Problems solved by technology

[0002] Animal and plant cell culture uses cell culture medium for stirring to provide nutrients for plant cells, so that they can get nutrients in the culture medium. Through stirring, the cells can be fully and evenly contacted with the culture medium, so as to achieve a better culture effect, and to reduce stirring The shear force of the impeller baffle agitator with a larger cut is used, but the agitator with the baffle structure will generate a large centrifugal force when the stirring speed is too fast, so that the liquid rotates along the inner wall of the container, which is easy to make the plant cell chloroplast Pressure is generated, which causes the rupture of the cell membrane, resulting in the loss of plant cell activity, and because the inner wall of the container is relatively smooth, the liquid is easy to fit and rotate during the rotation, so that the fluctuation range of the liquid horizontal position at the same horizontal position is limited, and it is not easy to be rotated Force directs upward agitation, resulting in less liquid mixing

Images