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Protection solution for nucleic acid in biological sample and application thereof

A nucleic acid protection and biological sample technology, applied in the field of nucleic acid preservation, can solve the problems affecting the production of virus sampling protection solution, low guanidine salt content, shortage of guanidine isothiocyanate, etc., to prevent degradation, reduce toxicity, and inhibit nuclease. active effect

Pending Publication Date: 2021-01-29
广州捷倍斯生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Like the sudden outbreak of the new crown pneumonia epidemic in 2020, it will lead to a serious shortage of guanidine isothiocyanate on the market, which will seriously affect the production of virus sampling protection solution. Sample nucleic acid protection solution is very necessary

Method used

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  • Protection solution for nucleic acid in biological sample and application thereof
  • Protection solution for nucleic acid in biological sample and application thereof
  • Protection solution for nucleic acid in biological sample and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A biological sample nucleic acid preservation solution, consisting of the following components in the following contents: guanidine isothiocyanate 60g / L, sodium lauryl sarcosinate 4g / L, disodium edetate 8g / L , ammonium chloride 80g / L, mercaptoethanol 3% (V / V), sodium citrate 3g / L and the water of balance.

Embodiment 2

[0032] A biological sample nucleic acid preservation solution, consisting of the following components in the following contents: urea 180g / L, 3-sulfopropyl tetradecyl dimethyl betaine 3g / L, dipotassium edetate 12g / L, ammonium sulfate 132g / L, triphosphine hydrochloride 2g / L, sodium citrate 7g / L and the water of balance.

Embodiment 3

[0034] A biological sample nucleic acid preservation solution, consisting of the following components in the following contents: urea 60g / L, guanidine isothiocyanate 60g / L, Triton X-100 3g / L, ethylenediaminetetraacetic acid 6g / L L, ammonium sulfate 70g / L, aluminum ammonium sulfate 30g / L, dithiothreitol 5g / L, sodium citrate 5g / L and the water of surplus.

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Abstract

The invention relates to a protection solution for nucleic acid in a biological sample and application thereof, belonging to the technical field of nucleic acid preservation. The protection solution for nucleic acid in the biological sample comprises the following components: a nuclease inhibitor, a surfactant, a penetrating agent, a chelating agent and a reducing agent. The protection solution ofthe invention can rapidly inhibit the activity of nuclease possibly existing in cells and an external environment, prevents nucleic acid degradation in a biological sample, and efficiently protects the nucleic acid of the biological sample; and the protection solution has a low guanidine salt content, so the problem that the production of virus sampling protection liquid is influenced due to shortage of guanidine salt on the market is avoided.

Description

technical field [0001] The invention belongs to the technical field of nucleic acid preservation, and in particular relates to a biological sample nucleic acid protection solution and its application. Background technique [0002] Nucleic acid is a biological macromolecular compound synthesized by many nucleotides. According to different chemical compositions, nucleic acid can be divided into ribonucleic acid (RNA for short) and deoxyribonucleic acid (DNA for short). Nucleic acid can exist stably in organisms, but it is easy to cause nucleic acid degradation due to the release of nucleases after three-dimensional. [0003] With the advancement of modern medical technology, there are more and more molecular disease diagnosis applications. As the target of molecular disease diagnosis, the demand for extraction and preservation of nucleic acid is also increasing. Therefore, it is particularly critical to ensure the stability of nucleic acid samples in clinical samples, such as...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/10
CPCC12N15/10
Inventor 卢秀劲
Owner 广州捷倍斯生物科技有限公司