Dual-purpose microfluidic sperm collecting tube and control method

Abstract

The invention belongs to the technical field of reproductive medicine detection, and discloses a dual-purpose microfluidic sperm collecting tube. A shell is cylindrical and hollow; a microfiltration membrane is arranged at the bottom of the shell; a first partition plate is arranged on the upper side of the microfiltration membrane, and a certain gap is reserved between the microfiltration membrane and the first partition plate; the first partition plate is semicircular and is arranged on one side in the shell; an inner shell is rotationally sleeved with the outer shell; a clamping plate is integrally arranged at the upper end of the inner shell; the outer wall of the inner shell is tightly attached to the interior of the outer shell; the cross section area of the inner shell is semicircular, and a second partition plate is integrally arranged at the bottom of the inner shell; a second partition plate is arranged on the upper side of the first partition plate and tightly attached to the first partition plate; and the outer wall of the inner shell is tightly attached to the interior of the outer shell. The dual-purpose microfluidic sperm collecting tube is simple and reasonable in structure, sperm collecting work can be rapidly completed, the working efficiency of workers is improved, and the labor intensity is reduced.

Description

technical field [0001] The invention belongs to the technical field of reproductive medicine detection, and in particular relates to a dual-purpose microfluidic sperm collection tube and a control method. Background technique [0002] At present, according to the statistics of the World Health Organization, infertility has become the third largest worldwide disease after tumors and cardiovascular and cerebrovascular diseases. There are about 230 million couples of childbearing age in China, among whom there are nearly 10 million infertile patients, and the number is increasing at a rate of hundreds of thousands every year. In recent years, due to environmental pollution, life pressure, and lifestyle changes, the incidence of male infertility has been increasing year by year. Intrauterine insemination (IUI), in vitro fertilization-embryo transfer (IVF-ET), intracytoplasmic sperm injection (ICSI) and other assisted reproductive technologies are currently the main technical me...

AI Technical Summary

Problems solved by technology

[0005] (1) The upstream method cannot distinguish between normal and abnormal sperm, which will lead to a large number of sperm with normal vitality but abnormal shape mixed into the final collected sperm, resulting in a low fertilization rate;

[0006] (2) After the upstream end, it is necessary to manually suck out the sperm suspension with better vitality in the upper layer, but if the operation is not done properly, it is easy to inhale the sperm and impurities in the lower layer;

[0007] (3) Repeated centrifugation steps in density gradient centrifugation lead to the generation of reactive oxygen species, which may greatly damage the DNA integrity of seemingly viable and morphologically normal sperm, thereby affecting sperm quality, resulting in low fertilization rates or abnormal embryonic development

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