Stem cell exosome microneedle patch for removing freckles and wrinkles and preparation method of stem cell exosome microneedle patch

A stem cell and exosome technology, used in microneedles, medical preparations with inactive ingredients, and medical preparations containing active ingredients, etc., can solve the problem of poor individual targeting, serious product homogeneity, and single active ingredients. It can reduce the deposition of scars and pigments, reduce melanin, and inhibit the activity of tyrosinase.

Active Publication Date: 2021-02-09
中科细胞科技(广州)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the microneedle patches on the market directly use stem cells or growth factors as the material solution, the active ingredient is single, the individual pertinence is not strong, the product effect is not good, and the product homogeneity is serious

Method used

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  • Stem cell exosome microneedle patch for removing freckles and wrinkles and preparation method of stem cell exosome microneedle patch
  • Stem cell exosome microneedle patch for removing freckles and wrinkles and preparation method of stem cell exosome microneedle patch
  • Stem cell exosome microneedle patch for removing freckles and wrinkles and preparation method of stem cell exosome microneedle patch

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1 Preparation of stem cell exosomes

[0049] 1. Isolation and culture of umbilical cord mesenchymal stem cells

[0050] Take 15-20cm long neonatal umbilical cord, require obstetrics and gynecology healthy full-term caesarean section fetus, pregnant women HBV antigen, anti-HCV antibody, anti-HIV antibody, anti-Treponema pallidum antibody, mycoplasma, anti-cytomegalovirus antibody and other tests are all negative. Treat within 6 hours, wash with PBS under sterile conditions until there is no blood, cut it longitudinally, remove the blood vessels and cut it into pieces of tissue with a size of 1mm×1mm×1mm, add 0.5ml of umbilical cord tissue suspension to a 75cm2 culture bottle, and add 10ml at the same time Serum-free complete medium, cultured in a 37°C, 5% CO2 incubator. Change the medium in half on the 3rd day and the full volume on the 7th day. Observe under an inverted microscope. When the cells grow to 80% confluent, digest with 0.05% trypsin and passage at a...

Embodiment 2

[0055] Example 2 Identification of stem cells and detection of exosomes

[0056] 1. Identification of umbilical cord mesenchymal stem cells

[0057] (1) Take 1 bottle of T175 bottle of umbilical cord mesenchymal stem cells with a confluence of 80% to 90% (cell number 1×10 7 ), digest with 5ml 0.05% trypsin for 3-5min; when most of the cells shrink and become round under the microscope, add 2mL serum-containing medium to stop the digestion, blow and beat the cells and transfer them to a 15mL centrifuge tube for centrifugation at 800rpm, centrifuge The time is 5 minutes;

[0058] (2) Cell preparation: Discard the supernatant, add 2mL PBS to suspend the pellet, and count the cells; 5 Distribute the cell suspension evenly into 1.5mL Ep tubes, centrifuge at 800rpm for 5min; discard the supernatant, add 100μL of PBS to the pellet to suspend;

[0059] (3) Antibody incubation: Add 1 μL of the corresponding flow-type antibody to each tube, incubate in the dark for 40 minutes, wash w...

Embodiment 3

[0064] Example 3 Preparation of Stem Cell Exosome Microneedle Patch

[0065] 1. Preparation of needle tip solution (exosome repair solution)

[0066] (1) Composition:

[0067] Exosomes (2.5×10 14 / L);

[0068] Astaxanthin (15g / L);

[0069] Trehalose (40g / L);

[0070] Chitosan (7.5g / L);

[0071] Bovine serum albumin (2g / L);

[0072] Phosphate buffer (0.015mol / L) was used to adjust the pH value to 7.2.

[0073] (2) The solution prepared in the above steps was mixed according to the formula to prepare an exosome repair solution, and then filtered through a 500-mesh cell sieve to obtain a microneedle tip solution.

[0074] 2. Preparation of needle solution

[0075] The needle solution was prepared by PVP K30 (2000g / L), PEG400 (20g / L), squalane (20g / L) and sterile water 500μl.

[0076] 3. Preparation of waterproof backing layer

[0077] (1) Backing layer formula: 4-8 parts of film material polyvinyl alcohol, 2-4 parts of thickener sodium carboxymethyl cellulose, 2-8 parts...

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Abstract

The invention discloses a stem cell exosome microneedle patch for removing freckles and wrinkles. The stem cell exosome microneedle patch comprises a microneedle and a waterproof backing layer for fixing the microneedle, wherein the microneedle is obtained by curing a needle tip solution and a needle body solution, the needle tip solution comprises a stem cell exosome, astaxanthin, trehalose, chitosan, bovine serum albumin and a phosphate buffer solution; and the needle body solution is prepared from PVP K30, PEG 400, squalane and sterile water. The microneedle patch disclosed by the inventioncan penetrate through a skin cuticle barrier, a novel administration path is provided for macromolecular drugs, and the problem of transdermal absorption of an exosome repair solution is solved. Themicroneedle patch can slowly release exosome repair solution, so that the absorptivity of an organism is improved. The microneedle patch has good puncture property, solubility and biological activity,is simple to operate and easy to store, and can be produced on a large scale.

Description

technical field [0001] The present invention relates to the field of stem cell microneedle cosmetology, and more specifically relates to a stem cell exosome microneedle patch for removing freckles and wrinkles and a preparation method thereof. Background technique [0002] Facial anti-aging is an increasingly popular topic in cosmetic surgery. The appearance of wrinkles and pigmentation is the most direct manifestation of facial aging. Therefore, wrinkle removal and freckle removal have also become topics that people pay more and more attention to. At present, medical cosmetology and surgical cosmetology are both temporary measures that treat the symptoms but not the root cause, with high risks, complications, side effects, and problems such as pain and bruising. Microneedle injection technology is favored by the majority of beauty lovers because of its minimally invasive, painless, quick recovery and good results. [0003] The skin, especially the stratum corneum, has a b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/28A61K31/122A61K9/70A61K9/00A61K47/26A61K47/36A61K47/42A61K47/02A61P17/00A61P17/18A61M37/00
CPCA61K35/28A61K31/122A61K9/7023A61K9/0021A61K47/26A61K47/36A61K47/42A61K47/02A61P17/00A61P17/18A61M37/0015A61M2037/0053A61M2037/0046A61K2300/00Y02A50/30
Inventor 唐忆琳华佩君陈巧林
Owner 中科细胞科技(广州)有限公司
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