SNP molecular marker for identifying production place of radix scutellariae and method and application thereof

A molecular marker and molecular marker technology, which can be used in biochemical equipment and methods, recombinant DNA technology, and microbial determination/inspection, etc. The effect of strong, high identification accuracy, easy to popularize and apply on a large scale

Active Publication Date: 2021-02-09
CHINA TRADITIONAL CHINESE MEDICINE +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the identification of origin of Scutellaria baicalensis mostly uses methods such as character identification and microscopic identification, relying on personal experience and subjective judgment, the accuracy is not high, and the technology is not scalable.

Method used

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  • SNP molecular marker for identifying production place of radix scutellariae and method and application thereof
  • SNP molecular marker for identifying production place of radix scutellariae and method and application thereof
  • SNP molecular marker for identifying production place of radix scutellariae and method and application thereof

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Embodiment 1

[0063] Sample information: Shaanxi-Shanxi-Shandong (HQ351, HQ381, HQ382, HQ391, HQ392, HQ471, HQ472, HQ491, HQ492), Liaoning-Jilin (HQ481, HQ482, HQ501, HQ502, HQ541, HQ542), Inner Mongolia (HQ361) were collected respectively , HQ362, HQ401, HQ402, HQ431, HQ432) area of ​​Scutellaria baicalensis. The specific information of the samples can be found in Table 1.

[0064] Table 1 Specific information of samples

[0065]

[0066] The steps to obtain the SNP molecular markers for identifying the origin of Scutellaria baicalensis are as follows:

[0067] 1. Sample DNA extraction The mCTAB method (modified CTAB method) was also used to extract DNA from the leaves of Scutellaria baicalensis. The specific extraction steps are as follows:

[0068] (1) Put 100 mg of fresh plant leaves into a 2.0 mL centrifuge tube, add a small amount of quartz sand and a magnetic bead at the same time, immerse the fresh leaves in liquid nitrogen for about 1 min, put them in a grinder and grind them...

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Abstract

The invention relates to an SNP molecular marker for identifying the producing place of radix scutellariae and a method and application thereof. The molecular marker comprises 20 SNP molecular markers, and the nucleotide sequences of the 20 SNP molecular markers are shown as SEQ ID NO.1 to SEQ ID NO.20. According to the SNP molecular marker for identifying the production place of the radix scutellariae, which is disclosed by the invention, the SNP marker is obtained on the genome level through a genomics technology, the radix scutellariae of which the production places are Shaanxi, Shanxi or Shandong can be rapidly and accurately identified by adopting the SNP molecular marker disclosed by the invention, the radix scutellariae with different production places is screened, the identification accuracy is high, the stability is high, and the SNP molecular marker is easy to popularize and apply in a wide range.

Description

technical field [0001] The invention belongs to the field of molecular biology molecular markers, in particular to a SNP molecular marker for identifying the origin of Scutellaria baicalensis and its method and application. Background technique [0002] Scutellaria baicalensis is a widely used traditional Chinese medicinal material. Its root is used as medicine. It is bitter in taste and cold in nature. Indications for febrile disease, upper respiratory tract infection, lung-heat cough, damp-heat jaundice, pneumonia, dysentery, hemoptysis, red eyes, restless fetus, high blood pressure, carbuncle, swollen boils and other diseases. The clinical antibacterial activity of Scutellaria baicalensis is better than that of Coptis chinensis, and it does not produce drug resistance. According to relevant statistics, 70% of Chinese patent medicines currently on the market contain Scutellaria baicalensis, such as commonly used Chinese patent medicines Qingkailing oral liquid, Shuanghuan...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12Q1/6869C12N15/11
CPCC12Q1/6869C12Q1/6895C12Q2600/156C12Q2535/122C12Q2537/165
Inventor 王继永郑司浩曾燕刘美娟尚兴朴赵莎王浩孙文静刘国城静一朱亚兵
Owner CHINA TRADITIONAL CHINESE MEDICINE
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