Polysaccharide SM-0.2M and anti-tumor product prepared from polysaccharide SM-0.2M
A technology of polysaccharides and products, which is applied to the polysaccharide SM-0.2M in raspberry polysaccharides and its preparation field, can solve problems that have not been studied in depth, and achieve the effect of enhancing phagocytosis and improving immunity
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[0051]Example 1
[0052]First, the extraction of crude polysaccharides
[0053]Raspberry jelly fruit is dried, crushed to remove seeds, add 1-10 times petroleum ether (boiling range 60~90℃) to defat, after removing petroleum ether, add 85% ethanol and reflux at 50~70℃ to remove monosaccharides and pigments. The filtered residue is extracted with 5~30 times pure water ultrasonically (power 50~70W, temperature: 50~90℃, treatment time: 60min~100min), centrifuge to take the supernatant, the filter residue is extracted twice according to the original conditions, and the filtrate is combined , Concentrate under reduced pressure to 1 / 4 of the original volume, add 3 to 5 times the volume of 95% ethanol, stand at 4 degrees Celsius for 24 hours, filter and collect the filter residue, and freeze-dry to obtain the crude raspberry polysaccharide.
[0054]2. Separation and purification of homogeneous polysaccharide components of raspberry
[0055]DEAE Sepharose Fast Flow packing is used for polar separation ...
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