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Screening method and application of anti-inflammatory active ingredient ethyl gallate in gallnut

A technology of ethyl gallate and active ingredients, which is applied in the field of medicine and can solve problems such as cervicitis that has not been reported in the application

Pending Publication Date: 2021-02-19
SHIHEZI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the screening of anti-inflammatory active ingredients in gallnut and the application of ethyl gallate in the treatment of cervicitis

Method used

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  • Screening method and application of anti-inflammatory active ingredient ethyl gallate in gallnut
  • Screening method and application of anti-inflammatory active ingredient ethyl gallate in gallnut
  • Screening method and application of anti-inflammatory active ingredient ethyl gallate in gallnut

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] LC-MS purification system separates gallnut components:

[0029] a, select the insects on the leaves of Rhus chinensis Mill., Rhuspotaninii Maxim. or Rhus punjabensis Stew.var.sinica (Diels) Rehd.etWils. gall as medicinal material of Galla;

[0030] b. Crumble the Galla gall medicinal material through a 60-mesh sieve, weigh 100g of medicinal material powder, and use 300mL distilled water as the solvent to reflux extract for 3 times, each extraction for 2 hours, and combine the extracts;

[0031] c. Use the X Bridge C18 chromatographic column to purify and prepare the combined extracts obtained in step b, and use the X Bridge C18 chromatographic column to purify and prepare the components in gallnut. The sample is eluted at a flow rate of 17mL / min, the injection volume is 20μL, the sample concentration is 1.5mg / mL, and A is used as 0.2% formic acid aqueous solution, B is acetonitrile as the mobile phase; the elution program is: 0-8min is 93%-80%A; 8-35min is 93%-80%A; 3...

Embodiment 2

[0041] Experimental materials and methods

[0042] Drugs and Reagents

[0043] The Galla gall medicinal material was purchased in Hanzhong, Shaanxi, and was identified as Rhus chinensis Mill. by Professor Han Bo (School of Pharmacy, Shihezi University); acetonitrile (chromatographic grade, Fisher Scientific); formic acid (excellent grade, Tianjin Guangfu Fine Chemical Research Institute); Burke's modified Eagle's medium (DMEM, Shanghai Gibco); fetal bovine serum (FBS, Biological Industries); PBS buffer (Solarbio); lipopolysaccharide (LPS, Sigma Chemical); 3-(4,5- Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, Solarbio); Dimethylsulfoxide (DMSO, Solarbio); Penicillin-streptomycin solution (Solarbio); Protease-EDTA digestion solution (T1320, Beijing Suo Laibao Technology Co., Ltd.); active oxygen detection kit (E004, Nanjing Jiancheng Bioengineering Research Institute); nitric oxide ELISA kit (Cat.#m1003096-C, Shanghai Enzyme Biotechnology Co., Ltd.); Tumor Necros...

Embodiment 3

[0051] Experimental study of ethyl gallate in the treatment of cervicitis:

[0052] Objective: To study the therapeutic effect of ethyl gallate on rats with cervicitis;

[0053] Method: 25% phenol mucilage was used to construct the colitis model, and after successful modeling, it was randomly divided into blank group, model group, positive drug group and ethyl gallate administration group; Score the objects, detect the cervical weight and visceral index, detect the expression of tumor necrosis factor α (TNF-α), epidermal growth factor (EGF) and epidermal growth factor receptor (EGFR) in rat serum, and evaluate the cervical Pathological sectioning of tissues;

[0054] Results: The vaginal opening of the rats in the model group was red and swollen, with viscous secretions flowing out, the coat color became worse, and the appetite decreased; compared with the model group, after drug treatment, the rats in the positive drug group and ethyl gallate group gradually recovered. Acti...

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Abstract

The invention relates to a screening method and application of anti-inflammatory active ingredient ethyl gallate in gallnut. The method is characterized in that all ingredients in the gallnut are separated through a HPLC-MS purification separation system, and the active ingredients for treating cervicitis in the gallnut are screened through in-vitro and in-vivo experiments. In-vivo pharmacologicalstudies show that the ethyl gallate has an obvious treatment effect on a cervicitis rat; the ethyl gallate can relieve the situation of red and swollen vagina of the cervicitis rat, reduce cervical secretions and inhibit the increase of cervical viscera indexes; pathological tissue section results show that the ethyl gallate can reduce infiltration of inflammatory cells, and accelerate repair ofcervical mucosal epithelium; and enzyme-linked immunosorbent assay (ELISA) tests show that the ethyl gallate can inhibit the expression of a tumor necrosis factor-alpha (TNF-alpha) of the cervicitis rat, and promote the secretion of an epidermal growth factor (EGF) and an epidermal growth factor receptor (EGFR).

Description

technical field [0001] The invention belongs to the field of medicine, and relates to a screening method of ethyl gallate, an anti-inflammatory active ingredient in gallnuts, and its application. Background technique [0002] The study of cervicitis and non-specific cervicitis belongs to the medical research field of sexually transmitted infection (STI) of unknown etiology. In recent years, the incidence of cervicitis has continued to rise, and the affected population is becoming younger and younger. Since the epithelium of the cervical canal mucosa is a single-layer columnar epithelium, the anti-infection ability is poor and infection is prone to occur. Cervical infection begins in the lower genital tract (vagina) and progresses to pelvic inflammatory disease, upper genital tract (uterus and fallopian tubes) and ascending abdominal infection. The most common clinical cervicitis is acute endocervical mucositis. If acute cervicitis is not diagnosed and treated in time or pa...

Claims

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Application Information

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IPC IPC(8): C12Q1/02A61K31/235A61P15/00A61P29/00
CPCG01N33/5055G01N33/5014A61K31/235A61P15/00A61P29/00G01N2500/10
Inventor 韩博王忠英王秋平杨文静刘永浩薛芮王钰莹于玮张华田星陈文
Owner SHIHEZI UNIVERSITY
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