Method and device for detecting instability of free nucleic acid microsatellite

A technology for microsatellite instability and instability, applied in the field of microsatellite locus detection, can solve the problems of instability, unstable detection of free nucleic acid microsatellites, and difficulty in detecting microsatellites, and achieve comprehensive and accurate detection and simple sampling. Convenient, fast-running effects

Active Publication Date: 2021-02-23
北京求臻医疗器械有限公司
View PDF7 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to overcome the deficiencies of the above-mentioned technical defects, the present invention provides a method and device for detecting micro

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method and device for detecting instability of free nucleic acid microsatellite
  • Method and device for detecting instability of free nucleic acid microsatellite
  • Method and device for detecting instability of free nucleic acid microsatellite

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0032] Example A method for detecting microsatellite instability of free nucleic acid

[0033] (1) Extraction and sequencing of plasma cfDNA samples: Plasma extraction kits were used to extract cfDNA in plasma samples. The plasma samples came from the peripheral blood of tumor patients. For specific operations, see the QIAamp Circulating Nuleacid Kit kit manual from QIAGEN, using Qubit4. 0 and dsDNA HS Assay Kit to quantify the extracted cfDNA.

[0034] (2) Library construction: end repair and add A tail at the 3' end; take 10-50ng cfDNA into a PCR tube, make up to 50 μL with Low TE, and add reagents according to Table 2 below.

[0035] Table 2

[0036] Vortex and mix well, microcentrifuge, set the following table 3 to set up the program and perform the reaction on the PCR machine.

[0037]

[0038] table 3

[0039](3) Adapter connection: Add the corresponding reagents into the system after the above reaction is completed according to Table 4 below.

[0040]

[0041]...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to the technical field of microsatellite site detection, in particular to a method and device for detecting instability of a free nucleic acid microsatellite. The device comprises an MSI site discrimination model used for performing information analysis of the instability state of the free nucleic acid microsatellite of a plasma sample, and the MSI site discrimination model comprises an MSI training set data preparation unit, a site model construction unit and a sample MSI state analysis unit. Compared with the prior art, a control sample is not needed, and the steps of experiment, sequencing and analysis of the control sample are omitted, so that the cost and the analysis complexity are reduced, the microsatellite instability of the whole tumor of the patient can becomprehensively detected through the peripheral blood of the patient, real-time monitoring can be realized, the operation time is short, and few resources are consumed; not only can insertion and deletion mutations of a microsatellite sequence be detected, but also mononucleotide mutations of the microsatellite sequence can be detected, the detection is more comprehensive and accurate, and the running speed of a single sample is about 10 times higher than that of double samples.

Description

technical field [0001] The invention relates to the technical field of microsatellite site detection, in particular to a method and device for detecting microsatellite instability of free nucleic acid. Background technique [0002] Colorectal cancer (CRC) is one of the most common malignant tumors, and its incidence rate ranks third among various malignant tumors in my country, accounting for fifth in the mortality rate of malignant tumors. Its occurrence is a complex process involving multiple genes and various factors, among which microsatellite instability (MSI) caused by mismatch repair function defect is an important mechanism of occurrence. Most of hereditary nonpolyposis colorectal cancer (HNPCC, also known as lynch syndrome) and 15% of sporadic colorectal cancer are caused by microsatellite instability. [0003] Microsatellite instability (MSI) refers to the phenomenon that when DNA mismatch repair (MMR) is abnormal, the replication errors of microsatellites cannot ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12Q1/6886C12Q1/6869G16B40/00G16B30/00G16B20/30
CPCC12Q1/6869C12Q1/6886C12Q2600/118C12Q2600/156G16B20/30G16B30/00G16B40/00C12Q2525/151C12Q2525/173C12Q2525/191C12Q2531/113C12Q2523/308C12Q2535/122
Inventor 孙大伟辛琳张腾龙陈静段小红师雅宁王东亮
Owner 北京求臻医疗器械有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap