Application of IgA2 detection reagent in preparation of KRAS mutant colorectal cancer diagnostic agent

A detection reagent and technology for colorectal cancer, applied in the field of biomedicine, can solve problems such as inability to dynamically detect multiple times, tumor metastasis, etc., and achieve large-scale screening and good specificity.

Active Publication Date: 2021-02-23
THE SIXTH AFFILIATED HOSPITAL OF SUN YAT SEN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In vivo detection trauma can easily cause tumor metastasis, and there is a problem that dynamic multiple detection cannot be performed

Method used

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  • Application of IgA2 detection reagent in preparation of KRAS mutant colorectal cancer diagnostic agent
  • Application of IgA2 detection reagent in preparation of KRAS mutant colorectal cancer diagnostic agent
  • Application of IgA2 detection reagent in preparation of KRAS mutant colorectal cancer diagnostic agent

Examples

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Embodiment 1

[0051] Example 1 Expression Analysis of Differential Immunoglobulin Subtypes

[0052] experimental method:

[0053] The present invention first collects the blood plasma of 49 healthy people and 56 colorectal cancer patients (32 of which are KRAS wild type and 24 of which are KRAS mutant) in the Sixth Affiliated Hospital of Sun Yat-sen University, and obtain and screen out differential immunoglobulin subgroups through the following steps: type:

[0054] 1. Collect 3-5cm whole blood from healthy people or patients with colorectal cancer through sodium citrate anticoagulant blood collection tubes;

[0055] 2. Centrifuge at a speed of 500g for 10 minutes, and the blood cells are precipitated and absorbed into the upper layer of plasma;

[0056] 3. Collect the plasma supernatant and centrifuge at 2000g for 15 minutes to remove the precipitated plasma sample;

[0057] 4. Aliquot and store in -80°C refrigerator;

[0058] 5. Perform the detection of Ruiboao protein chip (model QA...

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Abstract

The invention belongs to the field of biological medicines, and relates to application of a IgA2 detection reagent in immunoglobulin subtypes in preparation of a KRAS mutant colorectal cancer diagnosis or prognosis reagent / kit. Research finds that IgA2 in immunoglobulin subtypes is low in expression in KRAS mutant colorectal cancer, and has great difference and credibility. IgA2 is used as a colorectal cancer diagnosis biomarker, has the advantages of strong specificity and high sensitivity, is beneficial to improving the diagnosis and treatment level of colorectal cancer, and effectively prevents and treats colorectal cancer.

Description

technical field [0001] The invention belongs to the field of biomedicine, and relates to the application of an IgA2 detection reagent in the preparation of a KRAS mutation diagnostic reagent / kit and a colorectal cancer diagnostic reagent / kit. [0002] Background of the invention [0003] Colorectal cancer (CRC) is the most common malignant tumor of the digestive tract and the second most common cancer in the world, with its high morbidity and mortality ranking the third and fourth respectively. In recent years, with the improvement of people's living standards, changes in lifestyle and population aging, the incidence of colorectal cancer in my country has gradually increased, ranking third in the incidence of malignant tumors and fifth in the mortality rate in my country. Seriously threaten people's health and life. Early detection, early diagnosis, and early treatment are the key to the treatment of colorectal cancer. However, most colorectal cancers have no obvious symptom...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574G01N33/58G01N33/535G01N33/533
CPCG01N33/57419G01N33/57446G01N33/57484G01N33/582G01N33/581G01N33/533G01N33/535G01N2800/06G01N2800/7028
Inventor 刘瑞贤杨湘玲陈骏雄温创宇韦丽丽谭会柳李伟倩徐重刘焕亮
Owner THE SIXTH AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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