Preparation method and application of co-assembled nano-drug of double-ligand targeted synergistic control renin angiotensin system

Abstract

The invention discloses a preparation method and application of a co-assembled nano-drug of a double-ligand targeted synergistic control renin angiotensin system. The preparation method comprises thefollowing steps of dissolving telmisartan in dimethyl sulfoxide to obtain a telmisartan solution, and heating the telmisartan solution for later use; preparing a first polypeptide; adding a phosphatebuffer solution into the first polypeptide, adjusting the pH value of the first polypeptide by using a carbonate solution, and performing ultrasonic dissolution to obtain a first polypeptide solution;heating the first polypeptide solution until the first polypeptide solution is boiled, cooling, adding the telmisartan solution, uniformly mixing, and reacting in water to obtain co-assembled hydrogel; and finally, removing unreacted telmisartan through high-speed centrifugation, and preparing the co-assembled nano-drug after freeze-drying. According to the preparing method, cooperative regulation and control on a rennin angiotensin system are achieved, the synergistic effect of the nano-drug in myocardial infarction treatment is achieved, in addition, AT1R expression in a myocardial infarction area in the myocardial infarction process is up-regulated, therefore, the co-assembled nano-drug can enrich the myocardial infarction part, and the targeting performance of the new nano-drug is endowed.

Description

technical field [0001] The invention belongs to the technical field of nano-medicine, and in particular relates to a preparation method and application of a co-assembled nano-drug of a double-ligand targeted and coordinated regulation of the renin-angiotensin system. Uses in stem medicine. Background technique [0002] The renin-angiotensin system (RAS) in the human body plays an important role in the occurrence and development of myocardial infarction, and the imbalance of this system will accelerate the development of the disease. There are two action axes in this system, the negative action axis targeting angiotensin II receptor 1, and the positive action axis targeting Mas receptor. There have been two-axis studies in the past, such as the use of telmisartan and angiotensin 1-7 in the treatment of cardiovascular diseases, but in the treatment of myocardial infarction, there is no application for synergistic regulation of RAS dual receptors. In addition, due to The diff...

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Problems solved by technology

[0004] The present invention proposes a preparation method and application of a co-assembled nanomedicine that targets and coordinates the renin-angiotensin system with dual ligands, so as to solve the problem of lack of drugs for the coordinated regulation of RAS dual receptors in the treatment of myocardial infarction. Another purpose of the study is to study the use of the new nano-medicine to improve myocardial infarction

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