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Composition and application of saponin ⅶ and multiple myeloma clinical medicine

A technology for multiple myeloma and saponins of papaya, which is applied in the field of medicine, can solve problems that have not been studied, and achieve the effect of inhibiting cell proliferation, inhibiting cell proliferation, and improving sensitivity

Active Publication Date: 2022-01-11
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Preclinical studies have shown that PP7 has broad-spectrum antitumor activity, including lung cancer, nasopharyngeal cancer, liver cancer, breast cancer, oral cancer, colon cancer, etc., but it has not been studied in MM so far

Method used

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  • Composition and application of saponin ⅶ and multiple myeloma clinical medicine
  • Composition and application of saponin ⅶ and multiple myeloma clinical medicine
  • Composition and application of saponin ⅶ and multiple myeloma clinical medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] CCK-8 method was used to detect the synergistic effect on MM cells after combined use of barley saponin Ⅶ and bortezomib in different concentration ratios on MM cells ANBL6 cells.

[0031] Experimental steps:

[0032] (1) ANBL6 cells (professor Zhou Wen from the Cancer Institute of Central South University) grew to the logarithmic phase, resuspended the cells into a 15mL centrifuge tube, and blown and mixed well. Cells were diluted 10 times in a 1.5 ml centrifuge tube, and the cell density was calculated by using a cytometer.

[0033] (2) Set the well plate group without adding cells and only complete medium (RPMI-1640 medium + 10% BI fetal bovine serum + 1% double antibody) and the control group without adding drugs, only cells and complete medium, each The concentration was set in 4 replicate wells.

[0034] (3) Adding different concentration ratios of diploside VII and bortezomib (0μM+0nM, 0μM+5nM, 0μM+7.5nM, 0μM+10nM, 0.5μM+0nM, 0.5μM+5nM, 0.5μM+ Inoculation of c...

Embodiment 2

[0046] CCK-8 method was used to detect the synergistic effect on MM cells after combined use of different concentrations of barley saponin Ⅶ and bortezomib on MM cells ANBL6-BR cells.

[0047] Experimental steps:

[0048] (1) ANBL6-BR (professor Zhou Wen from the Cancer Institute of Central South University) cells grow to the logarithmic phase, resuspend the cells in a 15mL centrifuge tube, and blow and mix well. Cells were diluted 10 times in a 1.5 ml centrifuge tube, and the cell density was calculated by using a cytometer.

[0049] (2) Set the well plate group without adding cells and only complete medium (RPMI-1640 medium + 10% BI fetal bovine serum + 1% double antibody) and the control group without adding drugs, only cells and complete medium, each The concentration was set in 4 replicate wells.

[0050] (3) Adding different concentration ratios of diploside VII and bortezomib (0μM+0nM, 0μM+40nM, 0μM+80nM, 0μM+160nM, 0.5μM+0nM, 0.5μM+40nM, 0.5μM+80nM , 0.5μM+160nM, 1μ...

Embodiment 3

[0063] Using CCK-8 method to detect the effect of saponin Ⅶ and bortezomib on MM cells alone and in combination on primary CD138 in MM patients + Effect on cell viability.

[0064] Experimental steps:

[0065] (1) Bone marrow mononuclear cells from MM patients (from Hunan Cancer Hospital) were isolated with lymphocyte separation medium (purchased from Tianjin Haoyang Biological Products Technology Co., Ltd.).

[0066] (2) Sorting CD138 in mononuclear cells with CD138 magnetic beads + cell.

[0067] (3) CD138 + The primary cells were resuspended in a 15mL centrifuge tube and mixed by blowing. Cells were diluted 10 times in a 1.5 ml centrifuge tube, and the cell density was calculated by using a cytometer.

[0068] (4) Set the well plate group without adding cells and only complete medium (RPMI-1640 medium + 10% BI fetal bovine serum + 1% double antibody) and the control group without adding drugs, only cells and complete medium, each The concentration was set in 4 replica...

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Abstract

The invention discloses the composition and application of saponin VII and multiple myeloma clinical medicine. The invention uses the composition of Chinese medicine monomer saponin VII and clinical medicine bortezomib or doxorubicin for multiple myeloma to prepare Medicines to treat multiple myeloma. Compared with patricia saponin VII or multiple myeloma clinical drugs used alone, the present invention adopts saponin VII and bortezomib or saponin VII and doxorubicin in combination, which has a better effect of inhibiting cell proliferation . The composition greatly improves the sensitivity of multiple myeloma cells to bortezomib or doxorubicin, and is especially suitable for treating relapsed / refractory multiple myeloma patients.

Description

technical field [0001] The invention relates to a medicine for treating multiple myeloma, in particular to the composition and application of papaya saponin VII and clinical medicine for multiple myeloma. It belongs to the field of medical technology. Background technique [0002] Multiple myeloma (multiple myeloma, MM), the second most common hematological malignancy (13%), is a genetically complex clonal plasma cell malignancy, accounting for 1% of all newly diagnosed tumors. MM is prone to occur in middle-aged and elderly people. With the aging of the population at home and abroad, the incidence of MM is increasing year by year. Although new targeted therapies such as proteasome inhibitor bortezomib and some immunomodulators have emerged in recent years, the treatment remission rate of MM patients has increased significantly. However, cell drug resistance and disease recurrence caused by treatment resistance will make MM enter the refractory stage, which poses new chall...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/7048A61K38/05A61K31/704A61P35/00A61P7/00
CPCA61K31/7048A61K38/05A61K31/704A61P35/00A61P7/00A61K2300/00
Inventor 萧小鹃王海勤刘静梁龙胡磊
Owner CENT SOUTH UNIV