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Separation and purification of tylosin by using liquid thermal response polymer EO<20>PO<80>

A technology for separation and purification of tylosin, applied in sugar derivatives, sugar derivatives, organic chemistry, etc., can solve the problems of unfavorable industrial scale-up, residence, and long phase separation time, and achieve easy process application and phase formation The effect of short time and convenient operation

Active Publication Date: 2021-03-05
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The renewable two-phase extraction technology has unique advantages and good application prospects in terms of products, but the responsive polymer that forms a renewable two-phase system The synthesis method is relatively complicated, expensive, and not conducive to industrial scale-up. The phase formation and phase separation of the two responsive polymers take too long, and the process is not mature enough and currently only stays in the small-scale test stage, which greatly limits the renewable dihydrate phase system development

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1 Thermal Responsive Renewable EO 20 PO 80 A method for forward extraction of tylosin in a water / water two-phase system.

[0016] The tylosin fermentation broth is pretreated, filtered and centrifuged for later use, firstly add 1mLEO to a 10mL centrifuge tube 20 PO 80 , then add 5mL of tylosin fermentation broth to it, shake and mix evenly, adjust the pH of the solution to 9.0, and place it in a water bath at 55°C for 30 minutes to form a phase. After taking it out, use a centrifuge to Centrifuge at 6000rpm for 3min. 100 μL of the upper and lower phases were drawn with a pipette gun, diluted 10 times with ultrapure water to 1 mL, and the concentrations of tylosin in the upper and lower phases were respectively determined according to the known liquid phase detection method of tylosin. Tylosin in heat-responsive renewable EO 20 PO 80 The partition coefficient of forward extraction in the water / water two-phase system is 0.063.

Embodiment example 2

[0017] Implementation Case 2 Thermally Responsive Renewable EO 20 PO 80 A method for reverse extraction of tylosin in a water / water two-phase system.

[0018] The upper phase raffinate in the centrifuge tube of the implementation case 1 was removed, and added with EO 20 PO 80 Equal volume of water, shake and mix it evenly, adjust the pH to 4.5, and put it in a 45°C water bath for 30 minutes to form two phases, take it out, and centrifuge it at 6000rpm for 3min in a centrifuge. Use a pipette gun to absorb 100 μL of the upper and lower phases, dilute it 10 times to 1 mL with ultrapure water, and measure the concentrations of tylosin in the upper and lower phases respectively according to the known liquid phase detection method for tylosin. Tylosin in heat-responsive renewable EO 20 PO 80 The distribution coefficient in the water / water two water phases is the ratio of the concentration of the upper and lower phases. Under this condition, the distribution coefficient in the p...

Embodiment 3

[0019] Embodiment 3 thermoresponsive polymer EO 20 PO 80 recovery test.

[0020] Put the lower phase obtained by back-extraction in Example 2 in a 50°C water bath, let it stand for 30 minutes to remove the upper aqueous solution, and separate and purify the heat-responsive polymer EO 20 PO 80 The recovery rate was 99.53%.

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PUM

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Abstract

The invention relates to two-aqueous-phase separation and purification of tylosin by temperature induction through a thermal response polymer ethylene oxide-propylene oxide random copolymer aqueous solution. A method disclosed by the invention comprises the following steps of: uniformly mixing EO<20>PO<80> and tylosin fermentation liquor according to a volume ratio of 1:5, adjusting the pH value to 9.0, and forming a two-aqueous-phase system through temperature induction, so that tylosin in the fermentation liquor is transferred and enriched in lower-phase EO<20>PO<80>; removing the upper phase, adding water with the volume equal to that of the lower phase into the upper phase, and adjusting the pH value to 4.5, so that tylosin is transferred from the lower phase EO<20>PO<80> and enrichedin the upper phase aqueous solution through temperature induction, wherein separation and purification of tylosin are achieved through forward extraction and reverse extraction; and the recovery rateof the thermal response polymer reaches 99.53% through temperature adjustment.

Description

technical field [0001] The present invention relates to the technical field of two-phase extraction, more specifically, relates to the technical field of renewable two-phase extraction, especially refers to the use of a single temperature-sensitive polymer to construct a recyclable two-phase system in the separation and purification process of tylosin Process design and optimization in . Background technique [0002] The renewable two-water phase system is to use responsive polymers to construct a two-water-phase system, and to realize the repeated reuse of the two-water-phase system through the efficient recovery of the responsive polymer. [0003] Ethylene oxide (EO)-propylene oxide (PO) random copolymer (EOPO) is a heat-responsive polymer that can synthesize different turbidity by adjusting its hydrophilicity and hydrophobicity according to the ratio of ethylene oxide to propylene oxide. point of thermoresponsive polymers, which commonly include EO 20 PO 80 、EO 30 PO ...

Claims

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Application Information

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IPC IPC(8): C07H1/06C07H17/08
CPCC07H1/06C07H17/08Y02W30/62
Inventor 曹学君万俊芬王艳王振平张燕杨婷魏艳丽
Owner EAST CHINA UNIV OF SCI & TECH