Trichoderma solid fermentation method

Abstract

The invention provides a trichoderma solid fermentation method. The trichoderma solid fermentation method comprises the following steps: trichoderma strain activation, seed fermentation liquid preparation and porous model solid fermentation culture. The method adopts porous model solid fermentation to produce trichoderma spores, and uses a commonly used briquettes mold to make a solid substrate into a porous shape, increases contact area between the solid substrate per unit volume and air, improves fermentation conditions, and increases spore production of solid fermentation of trichoderma.

Description

technical field [0001] The invention belongs to the technical field of agricultural biology, and in particular relates to a method for solid fermentation of Trichoderma. Background technique [0002] Trichoderma ( Trichoderma spp.) belongs to the subphylum Deuteromycetes, Hyphosporium, from the order Stemophila, from the Stemaceae, and the genus Trichoderma, widely exists in nature, and is the most potential biocontrol fungus for plant diseases among fungi, which can prevent and control A variety of plant diseases, commercial Trichoderma preparations have appeared abroad. Trichoderma preparations are live fungal preparations, and live fungal spore powder is generally obtained through solid fermentation. In the study of solid fermentation, Trichoderma can grow through the solid substrate more easily and obtain higher spore production when cultured in triangular flasks or other small containers, but when using shallow pans for large-scale fermentation, Trichoderma usually o...

AI Technical Summary

Problems solved by technology

In the study of solid fermentation, Trichoderma can grow through the solid substrate more easily and obtain higher spore production when cultured in triangular flasks or other small containers, but when using shallow pans for large-scale fermentation, Trichoderma usually only It grows well on the surface of the solid substrate, and the internal culture substrate often causes insufficient fermentation of Trichoderma due to insufficient ventilation.

[0003] Prior art In the large-scale growth of Trichoderma, Trichoderma usually only grows on the surface of the solid substrate, and the internal culture substrate often makes the growth of Trichoderma poor due to insufficient ventilation, resulting in low spore production per unit volume of Trichoderma solid fermentation, which is now Some Trichoderma conventional large-scale solid fermentation, unable to achieve full deep fermentation

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