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Isolated xylaria sp. and application thereof in preparation of natural herbicides

A herbicide and fungus technology, applied in the field of microorganisms, can solve the problems of no literature reports on herbicidal activity, and achieve the effects of large yield, large product content, and simple fermentation conditions

Active Publication Date: 2021-03-12
HUBEI BIOPESTICIDE ENG RES CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no literature report on its herbicidal activity

Method used

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  • Isolated xylaria sp. and application thereof in preparation of natural herbicides
  • Isolated xylaria sp. and application thereof in preparation of natural herbicides
  • Isolated xylaria sp. and application thereof in preparation of natural herbicides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The acquisition of Xylaria sp.NH-1801 and its physiological and biochemical properties:

[0024] The fungus Xylaria sp.NH-1801 of the present invention is obtained by separation and purification from the soil in the suburbs of Wuhan City, Hubei Province. After the strain is fermented, it is extracted with ethyl acetate to obtain ethyl acetate extract, and the method of inhibiting seed germination is adopted. , found that ethyl acetate extract could completely inhibit Arabidopsis seed germination. After being cultured on the PDA plate for 1 week, the back of the plate is pale yellow, the front is white, the hyphae are thick and dense, the aerial hyphae are well developed, and can produce staghorn-shaped branches. Through morphological identification and ITS sequence comparison, the strain was identified as Xylaria sp.

[0025] So far, the applicant has obtained a strain of Xylaria sp., which was sent to the China Center for Type Culture Collection on November 13, 2020. ...

Embodiment 2

[0027] Preparation of the active compound in the fermentation product of Xylaria sp.NH-1801:

[0028] (1) Preparation of culture medium:

[0029] Potato Dextrose Agar Medium (PDA): Potato 200g / L, Glucose 20g / L, Agar 20g / L, natural pH value; Rice medium: Rice 80g, water 100ml, put into 500ml culture bottle, sterilized at 121 degrees Celsius for backup use.

[0030] (2) Preparation of seed liquid: Inoculate the slant strain Xylaria sp.NH-1801 in an eggplant-shaped bottle (PDA medium), culture at a constant temperature of 28°C for 7 days, and inoculate the spores with sterile water containing 0.1% Tween-80. Washed, prepared to a concentration of 1.0 × 10 7 cells / mL of bacterial suspension for later use.

[0031] (3) Fermentation culture: insert the bacterium suspension into the rice by the inoculum amount of 3% (ml / g) (inoculate 5 bottles altogether, rice consumption 400g), 28 ℃, static culture 8 days.

[0032] (4) Extraction and separation: add an equal volume of ethyl aceta...

Embodiment 3

[0035] The active compound in the fermentation product of Xylaria sp.NH-1801 and the hydrolyzate of the compound inhibit the germination of barnyardgrass seeds:

[0036](1) Preparation of reagents: Weigh compound 1 (19,20-epoxy-cytochalasin Q), compound 2 (21-deacetyl-19,20-epoxy-cytochalasin Q) and herbicide 2,4-D 2 mg respectively, and use Methanol was dissolved to 1 mg / ml. Then diluted with methanol compound to 200ppm, 100ppm, 50ppm, 25ppm, 12.5ppm. Add reagents of various concentrations into 12-well plates, 200ul per well, 3 duplicate wells for each concentration, add the same dose of pure methanol to the blank control, and evaporate the methanol for later use.

[0037] (2) Preparation of barnyardgrass seeds: Rinse barnyard grass seeds (commercially available) with running water for 4 hours, disinfect with potassium permanganate solution for 15 min, rinse with sterile water, dry, and add 4 barnyard grass seeds to each well , and then add 200ul of sterile water, and place...

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Abstract

The invention relates to the field of microorganisms, in particular to a separated Xylaria sp. strain and application thereof in preparation of natural herbicides. The preservation number of the Xylaria sp. is CCTCC NO: M2020734, and a fermentation product of the Xylaria sp. can inhibit seed germination. Wherein the effective component in the fermentation product is 19,20-epoxy-cyctochalasin Q, and the compound and the hydrolysate (21-deacetyl-19,20-epoxycyctochalasin Q) thereof can significantly inhibit the germination and growth of monocotyledonous plant seeds and dicotyledonous plant seeds.The Xylaria sp. NH-1801 is high in growth speed and simple in culturing conditions; the preparation method of the active compound is simple, the yield is high, and the potential development value isachieved.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to an isolated Xylaria sp. and its application in the preparation of natural herbicides. Background technique [0002] The discovery and large-scale application of herbicides is one of the symbols of agricultural modernization, and has made great contributions to ensuring the safety of global food production. With the long-term application of a large number of herbicides, it not only brings huge pressure to the environment, but also due to the continuous survival and evolution of weeds, the types of resistant weeds continue to increase and the resistance continues to increase. This requires the scientific community to continuously develop green herbicides. New herbicides. Utilizing biologically active metabolites in natural organisms to develop new biological herbicides is an important way and future development direction of herbicide research and development. Because such compounds ...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N43/90A01N63/30A01P13/00C12P17/18C12R1/645
CPCC12N1/14A01N43/90A01N63/30C12P17/188
Inventor 方伟刘芳张志刚吴兆圆柯少勇
Owner HUBEI BIOPESTICIDE ENG RES CENT
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