Method for evaluating quality of body fluid specimen
A body fluid sample and quality technology, which is applied in the field of quality evaluation of body fluid samples, can solve problems such as the degree of decomposition, the time and effort required, and the need for whole genetic data.
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Embodiment 1
[0179] Selection of reference miRNA capable of detecting degradation in whole blood coagulation
[0180] (Sample preparation for detection of deterioration due to temperature influence)
[0181] The blood of 7 blood collection tubes was collected from 3 healthy people respectively, and in the state of whole blood, one of the 7 tubes was left to stand at room temperature (23°C) for 0.5 hours (this was used as a reference condition), and the remaining 6 tubes were The branch was left still for 6 hours at each temperature of 4°C, 18°C, 20°C, room temperature (23°C), 28°C, and 30°C. After the time was up, centrifugation was performed respectively, and the obtained serum was divided into 300 μL each within 10 minutes after centrifugation, and stored in a freezer at -80°C.
[0182] (sample preparation for detection of deterioration caused by prolonged standing at room temperature)
[0183] The blood of 4 blood collection tubes was collected from 3 healthy people respectively. In ...
Embodiment 2
[0199] Detection of deterioration during coagulation of whole blood with various miRNAs
[0200] The deterioration of the quality of the bodily fluid sample may be determined by combining any two reference miRNAs instead of a single miRNA.
[0201] Existing amounts of hsa-miR-204-3p (SEQ ID NO: 1) and hsa-miR-4730 (SEQ ID NO: 2) in whole blood under the standard conditions of Example 1 and under the condition of standing at 30° C. for 6 hours. The respective presence of these miRNAs under each condition is as follows Figure 5 As shown, the difference in the amount of these two miRNAs under each condition is calculated, then as Figure 6 like that. as table 3 and Figure 5 As shown, hsa-miR-204-3p is an miRNA that shifts to a low value through the degradation of the samples generated in the whole blood state, and hsa-miR-4730 shifts to a high value through the degradation of the samples generated in the whole blood state Of the miRNAs present in undegraded samples, hsa-mi...
Embodiment 3
[0204] Selection of reference miRNA capable of detecting deterioration in serum state
[0205] (Sample preparation for detection of deterioration caused by prolonged standing at 4°C in serum state (modulation 1))
[0206] The blood of 4 blood collection tubes was collected from 3 healthy people respectively, and all were left still at room temperature (23° C.) for 0.5 hour, and then centrifuged to obtain serum. The serum obtained from one mouse was aliquoted into 300 μL each within 10 minutes after centrifugation, and stored in a freezer at -80°C (this was used as a reference condition). The serum obtained from the remaining 3 tubes was left standing at 4°C for 12 hours, 21 hours, and 24 hours. After the time was up, the serum was divided into 300 μL each and stored in a freezer at -80°C.
[0207] (sample preparation for detection of deterioration by standing in serum state (modulation 2))
[0208] Blood in 7 blood collection tubes was collected from 3 healthy people, and a...
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