Group of aroma-producing fungi in Paphiopedilum barbigerum Tang etWang roots and application

A technology of Paphillium root and small leaves, applied in the field of microorganisms

Active Publication Date: 2021-03-19
贵州省林业科学研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few reports on the isolation and screening of aroma-producing endophytic fungi from P.

Method used

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  • Group of aroma-producing fungi in Paphiopedilum barbigerum Tang etWang roots and application
  • Group of aroma-producing fungi in Paphiopedilum barbigerum Tang etWang roots and application
  • Group of aroma-producing fungi in Paphiopedilum barbigerum Tang etWang roots and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The separation of embodiment 1 endophytes

[0027] 1. Isolation of bacteria

[0028] Select 2-3-year-old root samples of robust Paphiopediphyllum microphylla, soak the rhizomes in soapy water for 2 hours, rinse them with running water for 3-5 hours, and gently scrape off the root hairs, root covers and other appendages with a scalpel on the ultra-clean workbench. objects, examined under a microscope, selected root segments with mycelium clusters, placed in a petri dish, rinsed with sterile water 3-5 times; first sterilized with 75% alcohol for 1 min, and then sterilized with 0.1% mercuric chloride solution 4-5min, wash 5 times with sterile water; cut the sterilized roots into 2-3cm small pieces, put them in a 6cm-diameter petri dish filled with sterile water, and gently use a scalpel and tweezers Scrape the root segment to free the mycelium cluster in the root segment cortex; culture at a constant temperature of 24°C, observe under the microscope after 24 hours, use a ...

Embodiment 2

[0031] The identification of embodiment 2 endophytes

[0032] (1) Morphological identification

[0033] 1. Identification method

[0034] a. Microstructural characteristics of colonies and hyphae

[0035] The bacterial strains XYDL040 and XYDL003 were inoculated in the middle of the PDA medium plate, a piece of sterile cover glass was picked up with tweezers, and inserted obliquely into the medium at an angle of 45 degrees at a certain distance from the inoculation point; when the hyphae When the cover glass grows to about 2 / 5, the cover glass is taken out, and the structural characteristics of the hyphae are observed under a microscope.

[0036] b. Diaphragm ultrastructure

[0037]1) Fixation of materials: Discard the culture medium and add electron microscope fixative solution to fix at 4°C for 2-4 hours, centrifuge the cells at low speed until mung bean-sized cell clumps can be seen at the bottom of the tube, wrap in 1% agarose, and use 0.1M phosphate buffer PB (pH7 .4)...

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Abstract

The invention discloses a group of aroma-producing fungi in Paphiopedilum barbigerum Tang etWang roots and application. The fungi are named as Tulasnella.sp XYDL003 and Tulasnella.sp XYDL040. The twostrains are preserved in the China Center for Type Culture Collection (CCTCC for short, Luojia hill, Wuchang, Wuhan, and postcode: 430072), and have the preservation numbers of CCTCC M 2020122 and CCTCC M 2020123. The aroma-producing fungi are separated from the root system of Paphiopedilum barbigerum Tang etWang and have rich fruity aroma; in aroma metabolites generated by the aroma-producing fungi, gamma-decalactone has strong coconut and peach fruity aroma; and the discovery of the fungi provides a new microbial resource for replacing plants to originally produce flavors and fragrances.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a group of fungi producing aroma in the root of Papudendron microphyllum and its application. Background technique [0002] Paphiopedilum barbigerum Tang et Wang belongs to Orchidaceae (Orchidaceae) Paphiopedilum (Paphiopedilum) perennial herbaceous plant, is included in the IUCN species red list, belongs to the IUCN endangered species, mainly distributed in Guizhou and northern Guangxi at an altitude of 800-1500m Limestone hills conceal rocky places or crevices. The distribution of P. microphyllum in Guizhou is also extremely rare, and belongs to the distribution of small populations. The distribution and specificity of mycorrhizal fungi affecting the distribution of orchids and thus the rarity of orchids has been widely discussed as a result of many studies. [0003] The sources of flavors and fragrances mainly include natural and chemical synthesis. Although th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P17/04C12P7/06C12P7/26C12P7/04C12P7/24C12P5/00C12P17/18C11B9/02C12R1/645
CPCC12P17/04C12P7/06C12P7/26C12P7/04C12P7/24C12P5/002C12P17/181C11B9/02Y02E50/10
Inventor 田凡廖小锋李从瑞罗在柒颜凤霞王莲辉姜运力罗红
Owner 贵州省林业科学研究院
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