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Method of detecting hfmd

A hand-foot-mouth disease and protein technology, which is applied in the detection of protein biomarkers in saliva, can solve the problems of uncontrollable disease transmission, inaccurate diagnosis and identification, misdiagnosis, etc.

Pending Publication Date: 2021-03-19
AGENCY FOR SCI TECH & RES
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, for diagnostic complications arising from insignificant or atypical clinical presentations, visual judgment inevitably leads to misdiagnosis
Thus, inaccurate diagnostic discrimination leads to inappropriate medical regimens or uncontrolled disease spread

Method used

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  • Method of detecting hfmd
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  • Method of detecting hfmd

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Experimental program
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Embodiment 1

[0069] 1. Materials and Methods

[0070] Complete salivary proteome profiling of saliva obtained from children with HFMD and healthy children using a reductive dimethylation chemical labeling approach combined with a high-resolution mass spectrometry-based quantitative proteomics technique.

[0071] The comprehensive protocol for recruiting HFMD patients from KK Women's and Children's Hospital and healthy volunteers from childcare centers was approved by the Ethics Committee of SingHealth CIRB (Ref. No.: 2012 / 448 / E) NUS-IRB (Ref. Code: B-14-273) support, and proceed according to its guidelines.

[0072] Clinicians assessed patients using clinical case definitions for fever, oral ulceration, and palmar and plantar skin lesions for HFMD. Clinically determined cases of HFMD were confirmed using viral genotyping. Healthy volunteers also tested negative for enteroviruses (see Figure 5 ).

[0073] Pooled saliva from laboratory-confirmed HFDM cases was compared to pooled saliva ...

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Abstract

The present invention relates to a method of detecting Hand-Foot-and-Mouth Disease (HFMD) in samples obtained from test subjects. In particular, the method relates to the detection of protein biomarkers in the saliva of these test subjects. In a preferred embodiment, the method comprising detecting in a saliva sample the presence and / or amount of at least one protein biomarker selected from the group consisting of PRX-IV, Legumain, SIL1 and CREG1, wherein the presence of the protein biomarker in the saliva is indicative of HFMD.

Description

technical field [0001] The present invention relates to a method for detecting Hand-Foot-and-Mouth Disease (HFMD) from a sample obtained from a test subject. In particular, the method relates to the detection of protein biomarkers in the saliva of these test subjects. Background technique [0002] HFMD is an acute, seemingly innocuous, usually mild and self-limiting, viral disease that afflicts millions of infants and young children each year in the Western Pacific Region. It is caused by human enterovirus A (HEV-A) from the Enterovirus genus. In recent years, coxsackievirus A16 (CA16) and enterovirus 71 (EV71) are the main pathogenic factors of HFMD (Wang and Liu, 2014). However, spikes in the number of cases caused by other HEV-A serotypes such as coxsackievirus A6 (CA6) have also been reported. HFMD is usually a self-limiting disease characterized by fever and papules, sometimes maculopapular, with rashes on the palms, soles, elbows, and trunk, and mouth sores. Howeve...

Claims

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Application Information

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IPC IPC(8): G01N33/563
CPCG01N33/6893G01N33/56983
Inventor 朱章汉张美珊
Owner AGENCY FOR SCI TECH & RES