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Fermentation method for increasing gain of plant growth-promoting rhizobacteria

A rhizosphere growth-promoting bacteria and fermentation method technology, applied in the field of fermentation to increase the yield of rhizosphere growth-promoting bacteria, can solve the problems of low yield of rhizosphere growth-promoting bacteria, and achieve the effect of increasing the yield

Pending Publication Date: 2021-03-23
SANYANG WUXI ECOLOGICAL AGRI DEV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the deficiencies of the prior art, the present invention provides a fermentation method for increasing the yield of rhizosphere growth-promoting bacteria, which has the advantage of increasing the yield of rhizosphere growth-promoting bacteria and solves the problem of low yield of rhizosphere growth-promoting bacteria

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A fermentation method for improving the amount of growth-promoting bacteria in the rhizosphere, comprising the following steps:

[0025] S1. The rhizosphere growth-promoting bacteria consist of the following raw materials: 10 parts of sterile water, 5 parts of vegetable seeds, 6 parts of ethanol, 2 parts of sodium hypochlorite, 20 parts of soil samples, 6 parts of organic fertilizer, 10 parts of agar, and the vegetable seeds are cucumbers;

[0026] S2. Use ethanol to disinfect the surface of the vegetable seeds, rinse it with sterile water and sprinkle it on the soil sample, the concentration of ethanol is 75%, and the purity of the sodium hypochlorite is 2%;

[0027] S3. Sodium hypochlorite and organic fertilizer are evenly sprinkled on the soil sample, and the vegetable seeds are cultivated for 20 days at a room temperature of 30° C.;

[0028] S4. Collect the root exudates produced after the vegetable seeds are cultivated in S3, freeze-dry and weigh the collected root...

Embodiment 2

[0034] A fermentation method for improving the amount of growth-promoting bacteria in the rhizosphere, comprising the following steps:

[0035] S1. The rhizosphere growth-promoting bacteria consist of the following raw materials: 12 parts of sterile water, 5 parts of vegetable seeds, 6 parts of ethanol, 2 parts of sodium hypochlorite, 22 parts of soil samples, 7 parts of organic fertilizer, 11 parts of agar, and the vegetable seeds are cucumbers;

[0036] S2. Use ethanol to disinfect the surface of the vegetable seeds, rinse it with sterile water and sprinkle it on the soil sample, the concentration of ethanol is 75%, and the purity of the sodium hypochlorite is 3%;

[0037] S3. Sodium hypochlorite and organic fertilizer are evenly sprinkled on the soil sample, and the vegetable seeds are cultivated for 20 days at a room temperature of 30° C.;

[0038] S4. Collect the root exudates produced after the vegetable seeds are cultivated in S3, freeze-dry and weigh the collected root...

Embodiment 3

[0044] A fermentation method for improving the amount of growth-promoting bacteria in the rhizosphere, comprising the following steps:

[0045] S1. The rhizosphere growth-promoting bacteria consist of the following raw materials: 14 parts of sterile water, 5 parts of vegetable seeds, 6 parts of ethanol, 2 parts of sodium hypochlorite, 23 parts of soil samples, 6 parts of organic fertilizer, 11 parts of agar, and the vegetable seeds are tomato;

[0046] S2. Use ethanol to disinfect the surface of the vegetable seeds, rinse it with sterile water and sprinkle it on the soil sample, the concentration of ethanol is 75%, and the purity of the sodium hypochlorite is 3%;

[0047] S3. Sodium hypochlorite and organic fertilizer are evenly sprinkled on the soil sample, and the vegetable seeds are cultivated for 20 days at a room temperature of 30° C.;

[0048] S4. Collect the root exudates produced after the vegetable seeds are cultivated in S3, freeze-dry and weigh the collected root ex...

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Abstract

The invention relates to the technical field of microorganisms, and discloses a fermentation method for increasing gain of plant growth-promoting rhizobacteria. The fermentation method comprises the following steps: S1, the plant growth-promoting rhizobacteria is prepared from the following raw materials: 10-20 parts of sterile water, 5-6 parts of vegetable seeds, 6-9 parts of ethanol, 2-3 parts of sodium hypochlorite, 20-30 parts of a soil sample, 6-9 parts of organic fertilizer and 10-14 parts of agar; According to the fermentation method for increasing gain of the plant growth-promoting rhizobacteria, vegetable seeds are selected and subjected to disinfection and sterilization treatment with ethanol and sterile water, and is cultured in an appropriate environment, so that the survival rate of the vegetable seeds, the number and nutrition content of root hairs after growth can be guaranteed, and the survival rate of bacteria is guaranteed; and a culture medium is obtained through thesterile water and agar after root secretions are obtained, floating liquid at rhizomes is diluted and then applied to the culture medium, so that the gain of the plant growth-promoting rhizobacteriacan be guaranteed, and the gain of plant growth-promoting rhizobacteria can be greatly increased.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a fermentation method for increasing the amount of growth-promoting bacteria in the rhizosphere. Background technique [0002] Rhizosphere growth-promoting bacteria generally refer to rhizosphere microorganisms that can directly or indirectly promote plant growth, increase crop yield, and prevent and control diseases and insect pests. Antagonism, advantage in the competition of nutrients in the rhizosphere, especially the competition for iron, can secrete phytohormones to promote plant growth, can induce plants to produce systemic resistance and secrete enzymes that degrade pathogenic microorganisms, etc. [0003] The yield of the existing technology of promoting growth-promoting bacteria in the rhizosphere is relatively low, which cannot meet the normal demand for the growth-promoting bacteria in the rhizosphere. Therefore, we propose a fermentation method to increase the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02A01G22/05A01C1/00A01C1/08
CPCC12N1/20C12N1/02A01G22/05A01C1/00A01C1/08
Inventor 武以军
Owner SANYANG WUXI ECOLOGICAL AGRI DEV CO LTD
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