Micro-droplet pH detection method based on pH test paper and Raman spectrum

Abstract

The invention belongs to the technical field of pH measurement, and particularly relates to a micro-droplet pH detection method based on pH test paper and Raman spectrum. According to the invention, the pH test paper and the laser confocal Raman spectrometer system are combined for use, so that the pH detection of micro-droplets is realized. The method specifically comprises the following steps: to-be-detected micro-droplets react with pH test paper, when the pH fluctuates, the color of the pH test paper changes accordingly, and Raman peak signals of the test paper correspondingly change; andspecific Raman peak signal intensity is acquired through a laser confocal Raman spectrometer system so as to judge the pH value of the to-be-detected micro-droplets. In combination with the amplification effect of an optical microscope in the Raman spectrometer system, the pH of a sample is measured through the Raman peak intensity change of the dye on the pH test paper, and the method has the advantages of being accurate, stable, resistant to color interference, capable of achieving size resolution and the like, can be used for pH detection of micro-droplets and has a good application prospect.

Description

technical field [0001] The invention belongs to the technical field of pH measurement, and in particular relates to a pH measurement method for microdroplets. [0002] technical background [0003] Suspended microdroplets are important special microreactors in atmospheric chemical reactions and have profound effects on many atmospheric processes. As one of the key parameters, the pH of microdroplets determines the chemical form, phase transition, distribution, and reactivity of the substances inside the droplets, which further affects chemical products, aerosol processes, regional climate, and public health. For example, increased acidity enhances the ability of droplets to penetrate the body, increasing their toxicity. The haze, acid fog and other climate problems caused by aerosols also seriously affect people's normal life. Therefore, to scientifically understand droplet chemistry, it is imperative to measure the pH of individual droplets. [0004] Indirect measurements...

AI Technical Summary

Problems solved by technology

However, they still suffer from inherent errors due to low water content, low ionic strength, inorganic ion doping and kinetic limitations

And due to the lack of direct measurement of microdroplet pH, the scientific analysis of microdroplet chemical processes is still limited.

[0005] The existing micro-droplet pH detection methods are mainly based on the Raman spectroscopy of ions or indicating functional groups in the micro-droplets, which can only detect micro-droplets with high ion concentrations.

However, other pH measurement methods, such as the peak intensity calibration method with dye and confocal light microscope, the color calibration method with pH test paper and mobile phone, and the degradation degree calibration method with pH sensitive dyes, etc., have complex operations, harsh detection conditions, and ion Concentration requirements are high, it is difficult to achieve the desired effect

Images