Kit for preparing exosome in serum or plasma

Abstract

The invention discloses a kit for preparing exosome in serum or plasma. The kit for for preparing the exosome in the serum or the plasma comprises a box body, wherein an upper cover is fixedly installed at the top of the box body; upper fixing blocks are fixedly connected to the bottom ends of the left side and the right side of the upper cover; connecting rods are fixedly installed at the bottomsof the upper fixing blocks; lower fixing blocks are fixedly installed at the positions, corresponding to the upper fixing blocks, of the top ends of the left side and the right side of the box body;fastening bolts are installed in the lower fixing blocks; a base is fixedly installed at the bottom of the box body; a motor is fixedly installed in the base; an output shaft of the motor penetrates through the bottom end of the box body so as to be rotatably connected with a fixing plate; the top end of the fixing plate is fixedly connected with a rotating basin; a sliding plate is fixedly installed in the middle of the outer side of the rotating basin; a plurality of fixing clamping grooves are fixedly formed in the bottom end of the inner side of the rotating basin; and a circular supporting plate is fixedly arranged on the inner side wall of the rotating basin and located above the fixing clamping grooves. The kit for for preparing the exosome in the serum or the plasma provided by theinvention is reasonable in structure, reagent tubes with different tube diameters can be conveniently clamped and fixed, and the convenience in operation and use is achieved.

Description

technical field [0001] The invention belongs to the technical field of kits, and more specifically relates to a kit for preparing exosomes in serum or plasma. Background technique [0002] Exosomes refer to small membrane vesicles (30-150nm) that contain complex RNA and proteins, and now they specifically refer to discoid vesicles with a diameter of 40-100nm; they are mainly derived from the invagination of lysosomal particles in cells Multivesicular bodies are released into the extracellular matrix after the fusion of the multivesicular outer membrane and the cell membrane, and exosomes naturally exist in body fluids, including blood, saliva, urine, cerebrospinal fluid, and milk. At present, people usually use methods such as ultracentrifugation, immunomagnetic beads, ultrafiltration, precipitation or kits to achieve the extraction and separation of exosomes. However, most of the existing exosome preparation kits in serum or plasma simply store the reagent tubes. However, ...

AI Technical Summary

Problems solved by technology

However, most of the existing exosome preparation kits in serum or plasma simply store the reagent tubes. However, due to their simple structure and fixed structure, reagent tubes with different diameters cannot be clamped and placed, and they are prone to shaking. , resulting in a small scope of application and inconvenient use

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