Application of nadph and vitamin E in combination for preparing medicine for preventing or treating liver injury
A vitamin and liver injury technology, applied in the field of biomedicine, can solve the problem of acute liver injury not improving, and achieve the effect of improving the survival rate of liver cells, reducing the level of MDA, and reducing the degree of reduction.
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experiment example 1
[0049] Experimental Example 1 Metabolic distribution of NADPH in various organs in the body
[0050] 1. Drugs and reagents
[0051] NaOH buffer solution with pH 8: Weigh an appropriate amount of NaOH and dissolve it in distilled water. After fully dissolving, add concentrated hydrochloric acid (analytical grade) to adjust the pH to 8;
[0052] Preparation of NADPH solution: Weigh 8 mg of NADPH and dissolve it in 10 ml of pH=8 NaOH buffer solution to obtain a NADPH solution with a concentration of 0.8 mg / ml.
[0053] 2. Experimental method
[0054] Ten ICR mice, male, 20-25 g, were randomly divided into two groups, the normal group (Ctrl) and the NADPH group (NADPH).
[0055] Before the experiment, the mice in each group were weighed, and the mice in each group were fasted for 24 hours. Then each group was treated as follows. Normal group: According to the body weight of the mice, 10ml / kg of animal body weight was injected intraperitoneally with normal saline. NADPH group: f...
experiment example 2N
[0057] Experimental example 2 NADPH combined with vitamin E on CCl 4 Effects of Induced Acute Liver Injury in Mice
[0058] 1. Reagents and animals
[0059] Vitamin E solution (VE solution): Dissolve 40, 80, 160 mg of vitamin E powder in 10 ml of 10% glycerol aqueous solution to prepare three VE solutions with concentrations of 4, 8, and 16 mg / ml, respectively.
[0060] NADPH solution: Dissolve the NADPH powders of 2, 4, and 8 in NaOH buffer to prepare three NADPH solutions with concentrations of 0.2, 0.4, and 0.8 mg / ml, respectively.
[0061] Olive Oil: Purchased from Piaget Group, Spain.
[0062] CCl 4 Solution: CCl 4 (Analytical pure) dissolved in pure olive oil to obtain CCl with a volume percentage of 0.35% 4 solution.
[0063] Male mice of ICR, with a weight range of 20-25 g, were purchased from Zhaoyan New Drug Research Center (Suzhou, China). Animals were housed in an environment with a temperature of 22±1°C and a humidity of 65±5% with a light / dark cycle of 12h...
experiment example 3N
[0107] Experimental Example 3 The effect of NADPH combined with vitamin E on L-02 cells
[0108] 1. Experimental materials
[0109] NADPH solution: Dissolve NADPH in sterile water for injection to prepare a 10mM (mmol / L) NADPH stock solution, filter and sterilize it with a sterile filter, and then pack and store at -80°C. When in use, the complete medium is used as the dilution reagent, and the 10 mM NADPH stock solution is diluted according to the volume ratio of the mother solution to the dilution reagent as 1:2000 to obtain a NADPH solution with a concentration of 5 μM (μmol / L).
[0110] VE solution: filter and sterilize the 10% glycerol aqueous solution with a sterile filter, and sterilize the vitamin E powder by irradiation under a UV lamp. Use the 10% glycerol aqueous solution to prepare the VE solution into a 100 mM VE stock solution. When in use, the complete medium is used as the dilution reagent, and the 100mM / L VE stock solution is diluted according to the volume r...
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