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Extraction system and method for flavonoid substances in pine needles

An extraction method and technology of flavonoids, which are applied in the field of extraction system of flavonoids in pine needles, can solve the problems of cumbersome operation and low extraction rate, and achieve the effects of accelerating penetration, convenient operation, and ensuring the amount of fermentation bacteria

Inactive Publication Date: 2021-04-02
深圳市得利鑫科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Pine needles are the main by-products of pine plants. Traditional pine needles are often used as chemical fertilizers or biomass fuels. However, pine needles have great medicinal value. The pine needles have sedative, anti-inflammatory, anti-oxidant, and immune-enhancing effects. The above effects are great Therefore, because the pine needles contain a large amount of flavonoids, the commonly used extraction methods of flavonoids are ultrasonic-assisted, chemical extraction, and biological enzyme extraction, but the above extraction methods are difficult to fully extract the flavonoids in pine needles. , and the operation is cumbersome, and the system made for the above extraction method is also cumbersome to operate and the extraction rate is low. Therefore, the existing technology needs further improvement

Method used

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  • Extraction system and method for flavonoid substances in pine needles
  • Extraction system and method for flavonoid substances in pine needles
  • Extraction system and method for flavonoid substances in pine needles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A method for extracting flavonoids in pine leaves, which is prepared by the following steps: (1) Wash and break pine leaves, add water, Inonotus obliquus suspension, Aspergillus oryzae suspension, butyric acid bacteria Bacterial suspension, yeast suspension, stirred and fermented at 35°C for 10h;

[0025] (2) Then add cellulase at 35°C, stir at 70r / min for 10min, cool to room temperature, then at 25kHz, sonicate for 20min, concentrate by rotary evaporator 2 to half of the original volume, add petroleum Ether, heated at 40°C, stirred at 70r / min for 2min, after cooling, added ethanol solution, stirred at 5°C for 3min, then stood still for 2h, centrifuged and took the supernatant to obtain the flavonoid extract.

[0026] In the described step (1), pine needles: water: Inonotus obliquus bacterial suspension: Aspergillus oryzae bacterial suspension: Butyricum bacterial suspension: the mass volume ratio of yeast bacterial suspension is 200g: 600ml: 5ml: 1ml : 1ml: 0.3ml; pin...

Embodiment 2

[0031] A method for extracting flavonoids in pine leaves, which is prepared by the following steps: (1) Wash and break pine leaves, add water, Inonotus obliquus suspension, Aspergillus oryzae suspension, butyric acid bacteria Bacterial suspension, yeast suspension, stirred and fermented at 38°C for 11h;

[0032] (2) Then add cellulase at 40°C, stir at 75r / min for 15min, cool to room temperature, then at 30kHz, sonicate for 25min, concentrate by rotary evaporator 2, concentrate to half of the original volume, add petroleum Ether, heated at 45°C, stirred at 75r / min for 3min, after cooling, added ethanol solution, stirred at 5.5°C for 4min, then stood still for 2.5h, centrifuged and took the supernatant to obtain the flavonoid extract.

[0033] In the described step (1), pine needles: water: Inonotus obliquus bacterial suspension: Aspergillus oryzae bacterial suspension: Butyricum bacterial suspension: the mass volume ratio of yeast bacterial suspension is 300g: 702ml: 8ml: 2ml ...

Embodiment 3

[0035] A method for extracting flavonoids in pine leaves, which is prepared by the following steps: (1) Wash and break pine leaves, add water, Inonotus obliquus suspension, Aspergillus oryzae suspension, butyric acid bacteria Bacterial suspension, yeast suspension, stirred and fermented at 40°C for 12h;

[0036] (2) Then add cellulase at 45°C, stir at 80r / min for 20min, cool to room temperature, then at 40kHz, sonicate for 30min, concentrate by rotary evaporator 2, concentrate to half of the original volume, add petroleum Ether, heated at 50°C and stirred at 80r / min for 5min, after cooling, added ethanol solution, stirred at 6°C for 5min, then stood still for 3h, centrifuged and took the supernatant to obtain the flavonoid extract.

[0037] In the described step (1), pine needles: water: Inonotus obliquus bacterial suspension: Aspergillus oryzae bacterial suspension: Butyricum bacterial suspension: the mass volume ratio of yeast bacterial suspension is 500g: 800ml: 10ml: 3ml ...

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Abstract

The invention relates to the field of pine needle processing, in particular to an extraction system and method for flavonoid substances in pine needles. The system comprises a fermentation device, a discharge port of the fermentation device communicates with a feed port of a rotary evaporator, a discharge port of the rotary evaporator communicates with a feed port of a petroleum ether tank, a discharge port of the petroleum ether tank communicates with a feed port of an ethanol tank, a discharge port of the ethanol tank communicates with a centrifuge, and a steam outlet of the petroleum ethertank communicates with a condenser. The system is high in extraction rate, convenient to operate and energy-saving.

Description

technical field [0001] The invention relates to the field of pine needle processing, in particular to a system for extracting flavonoids from pine needles and an extraction method thereof. Background technique [0002] Pine needles are the main by-products of pine plants. Traditional pine needles are often used as chemical fertilizers or biomass fuels. However, pine needles have great medicinal value. The pine needles have sedative, anti-inflammatory, anti-oxidant, and immune-enhancing effects. The above effects are great Therefore, because the pine needles contain a large amount of flavonoids, the commonly used extraction methods of flavonoids are ultrasonic-assisted, chemical extraction, and biological enzyme extraction, but the above extraction methods are difficult to fully extract the flavonoids in pine needles. , and the operation is cumbersome, and the system made for the above extraction method is also cumbersome to operate, and the extraction rate is low. Therefore,...

Claims

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Application Information

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IPC IPC(8): A61K36/15A61P25/20A61P29/00A61P39/06A61P37/04C12M1/12C12M1/02C12M1/00
CPCA61K36/15A61K2236/19A61K2236/53A61P25/20A61P29/00A61P37/04A61P39/06C12M23/02C12M23/34C12M27/02C12M29/04
Inventor 张滕飞蔡胜
Owner 深圳市得利鑫科技有限公司