Agrobacterium sp. DP3, microbial agent comprising Agrobacterium sp. DP3 and application in field of biological fertilizer preparation

A technology of Agrobacterium and bio-fertilizer, applied in soil conditioning materials, microbial-based methods, applications, etc., can solve the problems that plants cannot directly absorb and utilize

Active Publication Date: 2021-04-09
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The total phosphorus content of soil in my country is relatively high, but the available phosphorus content that can be directly absorbed and utilized by plants generally does not exceed 5% of the total phosphorus content. Most of the phosphorus fertilizers and Ca in the soil 2+ , Fe 3+ , Fe 2+ 、Al 3+ Phosphorus is combined to form insoluble phosphate, which cannot be directly absorbed and utilized by plants, making phosphorus the main factor restricting crop production

Method used

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  • Agrobacterium sp. DP3, microbial agent comprising Agrobacterium sp. DP3 and application in field of biological fertilizer preparation
  • Agrobacterium sp. DP3, microbial agent comprising Agrobacterium sp. DP3 and application in field of biological fertilizer preparation
  • Agrobacterium sp. DP3, microbial agent comprising Agrobacterium sp. DP3 and application in field of biological fertilizer preparation

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Example 1 Screening and Identification of Salt-tolerant Phosphate-Solubilizing Bacteria

[0052] 1. Configuration of culture medium

[0053] LB liquid medium: tryptone 5g / L, yeast extract 2.5g / L, sodium chloride 5g / L, deionized water 1000mL, pH=7.4.

[0054] LB solid medium: tryptone 5g / L, yeast extract 2.5g / L, sodium chloride 5g / L, agar 20g / L, deionized water 1000mL, pH=7.4.

[0055] PKO liquid medium: glucose 10g / L, tricalcium phosphate 5g / L, ammonium sulfate 0.5g / L, sodium chloride 0.2g / L, potassium chloride 0.2g / L, magnesium sulfate heptahydrate 0.3g / L, sulfuric acid Manganese 0.03g / L, ferrous sulfate 0.003g / L, yeast powder 0.5g / L, distilled water 1000mL, pH=7.0±0.2, high temperature and high pressure sterilization at 121℃ for 30min.

[0056] PKO liquid medium: glucose 10g / L, tricalcium phosphate 5g / L, ammonium sulfate 0.5g / L, sodium chloride 0.2g / L, potassium chloride 0.2g / L, magnesium sulfate heptahydrate 0.3g / L, sulfuric acid Manganese 0.03g / L, ferrous sulfate...

Embodiment 2

[0077] The saline-alkali tolerance test of embodiment 2 phosphate-dissolving bacteria

[0078] 1. Solution configuration

[0079] Configuration of different gradient salinity solutions: set the salinity to 0.1g / L, 0.5g / L, 1.0g / L, 1.5g / L, 3.0g / L, set the alkalinity to 7.2, 7.6, 8.0, 8.4, A total of 20 treatments. The salinity is prepared with sodium chloride, and the alkalinity is adjusted with hydrochloric acid and sodium hydroxide.

[0080] The configuration of the non-salinity LB liquid medium: tryptone 5g / L, yeast extract 2.5g / L, deionized water 1000mL, pH natural.

[0081] 2. Determination of the growth curve of DP3 under different salinity

[0082] Inoculate strain DP3 into liquid LB medium and cultivate to OD=0.1 (bacteria density is about 10 8 cells / mL) and stored in a 4°C refrigerator for later use.

[0083] Inoculate the bacterium liquid with OD=1.0 with 5% inoculation amount into the culture medium with different salinity and alkalinity containing 10% non-salini...

Embodiment 3

[0085] Corn germination experiment under the inoculation of embodiment 3 phosphorus-solubilizing bacteria DP3

[0086] Select large, round and plump corn seeds, soak them in 70% absolute ethanol for 2 minutes for disinfection, and rinse them with tap water. Cover each petri dish with filter paper, sow 10 corn seeds, inoculate 10 9 DP3 bacteria and irrigated with water with different saline-alkaline gradients. The salinity gradient is 0.1g / L, 0.5g / L, 1.0g / L, 1.5g / L, 3.0g / L, the alkalinity gradient is 7.2, 7.6, 8.0, 8.4, a total of 20 treatments. Two parallels were set up for each saline-alkaline gradient. Bacillus megaterium, which also has the ability to dissolve phosphorus, was purchased from the China General Microorganism Culture Collection Center (CGMCC) as a control. Observe how the corn seeds germinate after a week.

[0087] The results showed that the germination rate of corn seeds inoculated with DP3 could reach more than 70% at any alkalinity and salinity of 3.0g / L,...

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Abstract

The invention particularly relates to Agrobacterium sp. DP3, a microbial agent comprising the Agrobacterium sp. DP3 and application in the field of biological fertilizer preparation. Phosphate-solubilizing bacteria can convert insoluble phosphate into soluble phosphorus which can be used for improving the microenvironment of saline-alkali soil, increasing the phosphorus utilization rate of the soil and promoting the growth of crops. The invention aims to screen a novel strain for increasing the survival rate of the crops in the saline-alkali soil and provides the Agrobacterium sp. DP3. The strain has good salt resistance, phosphorus solubilization and crop growth promotion effects, and has a good effect when being applied to biological bacterial fertilizers and soil conditioners.

Description

technical field [0001] The invention belongs to the technical field of soil improvement engineering strains, and in particular relates to an Agrobacterium sp. DP3, its bacterial agent and its application in the field of biological fertilizers. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] According to the reports of the Food and Agriculture Organization of the United Nations (FAO) and the United Nations Educational, Scientific and Cultural Organization (UNESCO), the area of ​​saline-alkali land in the world is as high as 9.5438×10 8 hm 2 , soil salinization is a global environmental problem. It is estimated that saline-alkali land in the world increases by 1.0×10 6 ~...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05G3/80C09K17/14C09K101/00C12R1/01
CPCC12N1/20C05G3/80C09K17/14C09K2101/00
Inventor 张建庄林岚周璐璐胡振谢慧君梁爽王禄山
Owner SHANDONG UNIV
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