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T cell receptor for recognizing KRAS mutation and coding sequence thereof

A cell receptor and sequence technology, applied in genetically modified cells, cells modified by introducing foreign genetic material, animal cells, etc., can solve problems such as tumors and stimulate cell growth

Active Publication Date: 2021-04-13
XLIFESC LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Once it mutates, it will continue to stimulate cell growth and lead to tumorigenesis

Method used

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  • T cell receptor for recognizing KRAS mutation and coding sequence thereof
  • T cell receptor for recognizing KRAS mutation and coding sequence thereof
  • T cell receptor for recognizing KRAS mutation and coding sequence thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0151] Example 1 Cloning KRAS G12V Antigen Short Peptide-Specific T Cells

[0152] Peripheral blood lymphocytes (PBL) from healthy volunteers with genotype HLA-A1101 were stimulated with a synthetic short peptide VVGAVGVGK (SEQ ID NO: 9) (Beijing Saibaisheng Gene Technology Co., Ltd.). The short peptide was annealed with biotin-labeled HLA-A1101 to prepare pHLA haploids. These haploids were combined with PE-labeled streptavidin (BD Company) to form PE-labeled tetramers, and the tetramers and anti-CD8-APC double-positive cells were sorted. Sorted cells were expanded and subjected to secondary sorting as described above, followed by monoclonalization by limiting dilution. Monoclonal cells were stained with tetramers, and the double-positive clones screened were as follows: image 3 shown. The double-positive clones obtained through layers of screening still need to meet further functional tests.

[0153] The function and specificity of the T cell clone were further detected ...

Embodiment 2

[0155] Example 2 Obtaining the construction of the TCR gene and vector of the KRAS G12V antigen short peptide-specific T cell clone

[0156] with Quick-RNA TM MiniPrep (ZYMO research) extracted the total RNA of the antigen short peptide-specific and HLA-A1101-restricted T cell clones screened in Example 1. The cDNA was synthesized using clontech's SMART RACE cDNA amplification kit, and the primers used were designed at the C-terminal conserved region of the human TCR gene. The sequence was cloned into T vector (TAKARA) for sequencing. It should be noted that this sequence is complementary and does not contain introns. After sequencing, the sequence structures of the TCR α chain and β chain expressed by the double-positive clone are shown in Figure 1 and Figure 2, respectively. Figure 1a , Figure 1b , Figure 1c , Figure 1d , Figure 1e and Figure 1f They are the amino acid sequence of TCRα chain variable domain, the nucleotide sequence of TCRα chain variable domain,...

Embodiment 3

[0166] Example 3 Expression, refolding and purification of KRAS G12V antigen short peptide-specific soluble TCR

[0167]In order to obtain a soluble TCR molecule, the α and β chains of the TCR molecule of the present invention may only include their variable domains and part of the constant domains respectively, and a cysteine ​​residue is introduced into the constant domains of the α and β chains respectively To form an artificial interchain disulfide bond, the positions of the introduced cysteine ​​residues are Thr48 of TRAC*01 exon 1 and Ser57 of TRBC2*01 exon 1; the amino acid sequence and nucleotides of the α chain sequence as Figure 4a and Figure 4b As shown, the amino acid sequence and nucleotide sequence of its β chain are as follows Figure 5a and Figure 5b shown. The target gene sequences of the above-mentioned TCRα and β chains were synthesized and inserted into the expression vector pET28a+ (Novagene) respectively by the standard method described in "Molecul...

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Abstract

The invention provides a T cell receptor (TCR) capable of being specifically combined with a KRASG12V mutation (KRAS G12V) antigen. Short peptide of the mutation antigen is VVGAVGVGK and can form a compound with HLA A1101, and the TCR is specifically combined with the compound. The invention also provides a nucleic acid molecule encoding the TCR and a vector comprising the nucleic acid molecule. In addition, the invention provides a cell for transducing the TCR.

Description

technical field [0001] The present invention relates to the TCR and its coding sequence that can recognize short peptides derived from the KRAS G12V antigen. The present invention also relates to the KRAS G12V-specific T cells obtained by transducing the above TCR, and their role in the prevention and treatment of KRAS G12V-related diseases use. Background technique [0002] KRAS gene (p21 gene) is a murine sarcoma virus oncogene, about 35kb in length, located on chromosome 12, is a member of the ras gene family, and encodes the KRAS protein. The KRAS gene is classified as wild-type or mutant. Normally, wild-type KRAS controls pathways that regulate cell growth. Once it mutates, it will continue to stimulate cell growth and lead to the occurrence of tumors. The common mutation sites of the KRAS gene are located at the codons 12 and 13 of exon 2 of the KRAS gene, and G12V is one of the seven common mutation sites (Chin J Lab Med.November 2012, Vol.35, No. .11). The KRAS ...

Claims

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Application Information

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IPC IPC(8): C07K14/725C12N15/12C12N15/867C12N5/10C07K19/00A61K39/00A61P35/00A61P37/00
CPCC07K14/7051C12N15/86C12N5/0636A61P35/00A61P37/00C12N2740/15043C12N2510/00C07K2319/00A61K39/4611A61K39/4632A61K39/464464A61P37/02C12N2740/16043C07K16/32A61K39/0005C07K2317/622A61K2039/505A61K47/6811A61K47/6849A61K38/00C12N2501/2302C12N2501/2307
Inventor 胡静孙含丽
Owner XLIFESC LTD
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