Scaffold with adhesive for articular cartilage repair
A technology of articular cartilage and cartilage, applied in joint implants, joint implants, medical science, etc., can solve problems such as inability to treat large lesions, collapse, OCA disease spread to subchondral bones, etc.
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Embodiment 1
[0092] The pigs are divided into at least two groups in which there is at least one control group. Each test group was applied with the barrier composition, while the control group was not applied with the barrier composition. In all groups, cartilage defects developed in the load-bearing area of the medial femoral condyle of the knee joint.
[0093] In each test group, the barrier composition was applied to the subchondral bone. Multiple test panels can be generated to test various barrier compositions. In all groups, the same substrate was applied after any barrier composition. Conditions related to the application of the substrate and any top polymeric barrier over the substrate should be consistent in all test and control groups.
[0094] Testing was performed at one month and at additional time periods to determine whether the barrier composition prevented migration of subchondral components such as cells and fluid into the cartilage lesion. Such testing may include...
Embodiment 2
[0098] Scaffolds for testing in pigs were prepared as described below. A honeycomb porous collagen sponge (5 mm in diameter and 1 mm in thickness, Koken, Tokyo, Japan) was soaked in 25 μl of cold 0.3% neutralized collagen solution (Vitrogen, Cohesion Tech, Palo Alto, CA), and then incubated at 37°C 1 hour. The neutralized collagen solution solidifies to form an acellular scaffold composed of collagen gel within the sponge.
[0099] The engineered cell construct implanted with adhesive and sutures was prepared by harvesting a biopsy sample from porcine articular cartilage, mincing the biopsy sample, and dissolving it in a solution containing 100 μg / ml penicillin and 100 units / ml chain Ham's F-12 (F-12, Invitrogen) in 1.5 mg / ml collagenase (CLS 1, Worthington, Freehold, NJ) in Ham's F-12 (F-12, Invitrogen) digested on a rotator at 37°C 18 hours. Undigested tissue was removed using a cell mesh (70 μm mesh, BD Biosciences, Franklin Lakes, NJ). Isolated porcine articular chondr...
Embodiment 3
[0103] Five different engineered cell constructs were implanted into pigs and their performance compared. The 5 constructs are a) blank defect control ("blank"); b) acellular scaffold control ("scaffold") implanted with adhesive and suture; c) engineered implanted with adhesive and suture cell constructs (“cell constructs”); d) engineered cell constructs implanted with only adhesives (“glued cell constructs”); and e) implanted on subchondral bone using only sutures Engineered cell constructs ("stitched cell constructs").
[0104] Two rounds of surgery were performed in pigs. Protocols for animal studies were approved by the Animal Care and Use Regulatory Committee of Charles River Laboratories (Worcester, MA). Sixteen castrated male pigs (Micro-Yucatan, Charles River Laboratories) aged 12 to 15 months and weighing 30 to 45 kg were acclimatized for more than one week prior to the first surgery. With 0.04 mg / kg atropine sulfate (Patterson, Devens, MA), 0.55 mg / kg butorphanol ...
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