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Drug combination and application thereof

A drug and combination technology, applied in metformin hydrochloride and union combination preparations and its application in the preparation of antibacterial drugs, application of hyperglycemia drugs, drug combination and its application field, can solve the problem of no effective treatment Bacterial infectious diseases and other problems, to achieve the effect of reducing high cytotoxicity and potential discoloration defects, good antibacterial effect, and improving antibacterial efficacy

Active Publication Date: 2021-04-20
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the prior art, there is no effective treatment of bacterial infectious diseases associated with diabetes or hyperglycemia

Method used

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  • Drug combination and application thereof
  • Drug combination and application thereof
  • Drug combination and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1: minimum inhibitory concentration (MIC) and bactericidal concentration (MBC) test

[0038] The test solution was silver nitrate (AgNO) containing 0, 3.2 (w / v)%, 6.4 (w / v)% Met 3 ) solution (AgNO 3 The solution concentration is 0, 5, 10, 20, 40, 80, 160, 320, 640 μg / mL). Prepare 2.56 mg / mL AgNO with sterile double distilled water 3 stock solution and 25.6% (w / v) Met stock solution. Test AgNO alone 3 or the MIC of Met, add 50 μL AgNO 3 The stock solution or Met stock solution and 50 μL sterilized double-distilled water were added to a 96-well plate and serially double-diluted with sterilized double-distilled water. Take the Enterococcus faecalis (ATCC) suspension with an absorbance value of 1 at 600nm, and serially dilute it to 10 in the double-concentration BHI medium / BHIG medium (additional 25mM glucose is added to the BHI medium). 6 CFUs / mL, take 100 μL of the diluted bacterial solution and add to the above 96-well plate, and incubate anaerobically a...

Embodiment 2

[0042] Embodiment 2: colony forming unit count experiment

[0043] In the double concentration BHI medium / BHIG medium (the concentration of each component in the medium is doubled), the Enterococcus faecalis suspension when the absorbance value at 600nm was 1 was diluted to 2×10 4 CFUs / mL, mix 500 μL diluted Enterococcus faecalis suspension with 500 μL AgNO-containing 3 mixed with Met solution to make AgNO 3 The final concentration of Met was 40 μg / mL, and the final concentration of Met was 3.2 (w / v)% and 6.4 (w / v)%. After mixing evenly, incubate at 37°C in the dark for 24 hours. After 24 hours, 10 μL of each was evenly spread on a BHI agar dish after 10-fold serial dilution, and cultured anaerobically at 37°C for 24 hours in the dark. The negative control group was co-incubated with bacterial solution and sterilized double distilled water, and the positive control group was co-cultured with bacterial solution and CHX, with a final concentration of 2 (w / v)% CHX. The above ...

Embodiment 3

[0045] Embodiment 3: dynamic antibacterial experiment

[0046] Take the Enterococcus faecalis suspension with an absorbance value of 1 at 600nm, and dilute it to 2×10 in double-concentration BHI medium / BHIG medium (the concentration of each component in the medium is doubled). 8 CFUs / mL. 700 μL AgNO 3 Add 700 μL diluted Enterococcus faecalis suspension to the mixed solution with Met to make AgNO 3 The final concentration of Met was the same as that in the above-mentioned Example 2, and after mixing evenly, they were incubated in a constant temperature incubator at 37° C. in the dark. At 2, 4, 6, 8, and 10 hours, take 200 μL and read the absorbance at 600 nm with a microplate reader. The negative control group was co-incubated with bacterial liquid and sterilized double distilled water, and the positive control group was co-incubated with bacterial liquid and CHX. The above operations were carried out under sterile conditions protected from light. Repeat 6 times.

[0047]...

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Abstract

The invention provides a drug combination. The drug combination is prepared from the following substances as active ingredients: metformin hydrochloride and silver; and the active ingredients are prepared together or separately for compatible use, simultaneous use or separate use. The drug combination is reasonable and efficient in drug combination, not only can improve the antibacterial curative effect, but also can reduce adverse reactions and toxic and side effects, and has incomparable superiority compared with single use of one of drugs. In addition, the antibacterial effect in a high-glucose environment is good, and toxic and side effects are small. The invention also provides application of the drug combination in preparation of antibacterial drugs, and particularly relates to application in preparation of drugs for prevention or treatment of bacterial infectious diseases of patients with diabetes and hyperglycemia.

Description

technical field [0001] The patent of the present invention relates to the field of pharmaceutical technology, especially to a drug combination and its application, specifically to the preparation of metformin hydrochloride and Union Union and its application in the preparation of antibacterial drugs and in the preparation of drugs for the treatment of hyperglycemia associated with diabetes Applications. Background technique [0002] Biguanide salt is a broad-spectrum fungicide, which can be divided into alkyl biguanide salts (metformin hydrochloride, biguanide octyl acetate), aryl biguanide salts (chlorhexidine) and polymeric biguanide salts (polyhexamethylene biguanide salts). Salt), etc., are widely used in chemical medicine, food, daily chemical and other fields. The configuration and alkyl chain length of biguanide compounds have a significant impact on the bactericidal performance. N 1 , N 5 Compounds substituted by aromatic alkanes have more extensive and effective...

Claims

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Application Information

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IPC IPC(8): A61K33/38A61K31/635A61P3/10A61P29/00A61P31/04A61K31/155
Inventor 范伟范兵吴旭英
Owner WUHAN UNIV
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