Glycosylated protein preparation method based on deep eutectic solvent

A glycosylated protein and deep eutectic solvent technology, which is applied in the field of protein processing, can solve the problems of poor emulsification ability and emulsification stability of glycoproteins, and achieve the effects of good emulsification activity, improved functional properties, and strong operability

Inactive Publication Date: 2021-04-20
DALIAN POLYTECHNIC UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The invention provides a method for preparing glycosylated protein using a deep eutectic solvent to solve the problem of poor emulsification ability and emulsification stability of the obtained glycoprotein in the traditional Maillard reaction

Method used

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  • Glycosylated protein preparation method based on deep eutectic solvent
  • Glycosylated protein preparation method based on deep eutectic solvent
  • Glycosylated protein preparation method based on deep eutectic solvent

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Embodiment 1

[0037] A method for preparing glycoprotein with a deep eutectic solvent, through the following process steps:

[0038] S1. Preparation of deep eutectic solvent: Mix choline chloride and glucose (Glucose, G) with a molar mass ratio of 1:1 in a beaker, stir at 200 rpm, and incubate in an oil bath at 90°C for 5 hours to obtain a uniform Transparent deep eutectic solvent (DES);

[0039] S2. Preparation of glycosylated protein: Dilute the DES prepared in S1 with PBS (0.01M, pH=7) into a 60% (w / w) DES solution, and mix thoroughly with a vortex mixer; then dilute the diluted DES The solution (5g) was incubated with bovine serum albumin (BSA, 0.25g) in an oil bath at 100°C for 2 hours to obtain glycosylated bovine serum albumin (GBSA);

[0040] S3. Preparation of glycosylated protein by traditional wet heat method (control group): Dilute 2.82g of glucose with 2mL of PBS (0.01M, pH=7), mix thoroughly; then mix with 0.25g of BSA, and place in an oil bath at 100°C Incubate for 2 hours to...

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Abstract

The invention provides a glycosylated protein preparation method based on a deep eutectic solvent, wherein the preparation method comprises the steps: S1, preparing the deep eutectic solvent: mixing and stirring choline chloride and glucose in a molar mass ratio of 1:1-1.5:1, to obtain uniform and transparent DES; and S2, preparation of glycosylated protein: diluting the DES prepared in the step S1 by using PBS according to a ratio of 5:2-2:1 m / m, fully mixing, mixing with BSA according to a ratio of 20:1-25:1 m / m, and carrying out oil bath at the temperature of 90-100 DEG C for 1.5-2.5 hours to obtain the GBSA. The glycosylation degree of the prepared protein is high, the emulsifying activity and emulsifying stability of the protein are good, the functional characteristics of glycoprotein are greatly improved, and the method is simple in process, high in operability and suitable for industrial production. The deep eutectic solvent has the advantages of biodegradability, no toxicity, good solubility, easy separation of reaction products and the like, and is suitable for food and drug application.

Description

technical field [0001] The invention relates to the technical field of protein processing, more specifically, to a method for preparing glycosylated protein with a deep eutectic solvent. Background technique [0002] As a functional additive, protein has the advantages of being non-toxic, harmless, nutritious, and natural compared with traditional chemically synthesized additives. Studies have shown that it is difficult for protein products to have multiple functional properties, such as stability, emulsification, foaming, etc., and cannot meet the requirements of all food processing, but what is required in production is to have special functions or a balance point of centralized functions The product. Therefore, in order to expand the application range of proteins, modification is the leading direction for the future development of proteins. [0003] In recent years, researchers have studied protein modification to improve the physicochemical and functional properties of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08H1/00
Inventor 宋亮赵珂瑶付晶晶徐献兵孙丛白长军姜鹏飞
Owner DALIAN POLYTECHNIC UNIVERSITY
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