Extraction process optimization method and rapid extraction method of poison mushroom alpha-amatoxin

A technology of amanita toxin and extraction process, which is applied in the field of detection of poisonous mushroom toxins, can solve the problems affecting the detection process efficiency, low efficiency, slow speed, etc., and achieve the effect of short extraction time and high efficiency

Pending Publication Date: 2021-04-23
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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Problems solved by technology

[0003] The detection of poisonous mushroom toxins needs to be extracted through pre-treatment, and the extraction technology greatly affects the efficiency of the entire detection process
At present, the methods for extracting amanita toxin from poisonous mushrooms are mainly shaking table method and ultrasonic method, but the shaking table extraction method has low efficiency and slow speed, and needs to be extracted for at least 24 hours, which seriously affects

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  • Extraction process optimization method and rapid extraction method of poison mushroom alpha-amatoxin
  • Extraction process optimization method and rapid extraction method of poison mushroom alpha-amatoxin
  • Extraction process optimization method and rapid extraction method of poison mushroom alpha-amatoxin

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Embodiment 1

[0026] The present invention is further illustrated by taking the deadly mushroom Amanita exitialis as an example.

[0027] 1. Materials and methods

[0028] 1.1 Material preparation: Fresh deadly Amanita fruiting body samples were dried in an oven at 50°C, crushed into powder and passed through a 100-mesh sieve, mixed thoroughly and then dried to obtain dry powder samples of poisonous mushrooms, which were stored for later use.

[0029] 1.2 Preparation of α-amanitin standard stock solution: α-amanitin standard stock solution was provided by the Fungal Laboratory of College of Life Sciences, Hunan Normal University. Accurately weigh 1.00 mg of α-amanitin standard substance, dissolve it in ultrapure water to 5.00 mL, and mix well to obtain α-amanitin standard stock solution. The α-amanitin standard stock solution has a concentration of 200.00 μg / mL and is stored at -20°C in the dark for future use.

[0030] 1.3 Preparation of toxin crude extract of poisonous mushroom fatal am...

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Abstract

The invention discloses an extraction process optimization method and a rapid extraction method of poison mushroom alpha-amatoxin. An ultrasonic extraction method is adopted to extract alpha-amatoxin, firstly, five test factors are screened through a single factor analysis test, then, a multi-factor multi-level test is designed through a response surface center combination method, the content of alpha-amatoxin is determined through an HPLC method, the extraction yield of alpha-amatoxin is calculated, optimal extraction process conditions of poison mushroom alpha-amatoxin are determined by integrating all experiment results, and then experiments are carried out for verification, so that the rapid and efficient ultrasonic extraction method of the poison mushroom alpha-amatoxin is finally obtained. The method is short in extraction time and high in efficiency.

Description

Technical field: [0001] The invention relates to the field of poisonous mushroom toxin detection, in particular to an extraction process optimization method and a rapid extraction method of poisonous mushroom α-amanitin. Background technique: [0002] Poisonous mushrooms are a general term for a class of mushrooms that can produce poisoning reactions after being eaten by humans or animals, and cause death in severe cases. Poisonous mushroom poisoning is a type of food poisoning with a high incidence rate and extremely serious consequences in China over the years. The fatality rate is as high as 21.24%, accounting for 35.57% of the deaths from food-borne poisoning. It has become a type of food safety accident with the highest fatality rate in my country. . Among them, the highly poisonous mushrooms containing amanitin have the highest poisoning mortality rate, accounting for more than 90% of all poisonous mushroom mortality rates. There are many kinds of poisonous mushrooms ...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 邓旺秋刘萌张成花李挺李泰辉
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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