Extraction process optimization method and rapid extraction method of poison mushroom alpha-amatoxin

Abstract

The invention discloses an extraction process optimization method and a rapid extraction method of poison mushroom alpha-amatoxin. An ultrasonic extraction method is adopted to extract alpha-amatoxin, firstly, five test factors are screened through a single factor analysis test, then, a multi-factor multi-level test is designed through a response surface center combination method, the content of alpha-amatoxin is determined through an HPLC method, the extraction yield of alpha-amatoxin is calculated, optimal extraction process conditions of poison mushroom alpha-amatoxin are determined by integrating all experiment results, and then experiments are carried out for verification, so that the rapid and efficient ultrasonic extraction method of the poison mushroom alpha-amatoxin is finally obtained. The method is short in extraction time and high in efficiency.

Description

Technical field: [0001] The invention relates to the field of poisonous mushroom toxin detection, in particular to an extraction process optimization method and a rapid extraction method of poisonous mushroom α-amanitin. Background technique: [0002] Poisonous mushrooms are a general term for a class of mushrooms that can produce poisoning reactions after being eaten by humans or animals, and cause death in severe cases. Poisonous mushroom poisoning is a type of food poisoning with a high incidence rate and extremely serious consequences in China over the years. The fatality rate is as high as 21.24%, accounting for 35.57% of the deaths from food-borne poisoning. It has become a type of food safety accident with the highest fatality rate in my country. . Among them, the highly poisonous mushrooms containing amanitin have the highest poisoning mortality rate, accounting for more than 90% of all poisonous mushroom mortality rates. There are many kinds of poisonous mushrooms ...

AI Technical Summary

Problems solved by technology

[0003] The detection of poisonous mushroom toxins needs to be extracted through pre-treatment, and the extraction technology greatly affects the efficiency of the entire detection process

At present, the methods for extracting amanita toxin from poisonous mushrooms are mainly shaking table method and ultrasonic method, but the shaking table extraction method has low efficiency and slow speed, and needs to be extracted for at least 24 hours, which seriously affects the rapid diagnosis and treatment of poisonous mushroom poisoning. effective treatment

Ultrasonic extraction has been applied to the toxin extraction of poisonous mushrooms many times in recent years because of its advantages of high extraction efficiency, short time, wide adaptability, simplicity, less impurities in the extract, and easy separation and purification of active ingredients. However, related extraction process optimization has not been reported yet.

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